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Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus
Wang, Xiao-Jie1,2; Yu, Ren-Cheng1; Luo, Xuan1; Zhou, Ming-Jiang1; Lin, Xiang-Tian3
2008-07-01
发表期刊TOXICON
ISSN0041-0101
卷号52期号:1页码:55-61
文章类型Article
摘要Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 x 10(2) mouse unit (MU) Per gram Of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus Vibrio, and the others were related to genus Shewanella, Marinomonas, Tenacibaculum and Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in N. semiplicatus. (C) 2008 Elsevier Ltd. All rights reserved.; Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 x 10(2) mouse unit (MU) Per gram Of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus Vibrio, and the others were related to genus Shewanella, Marinomonas, Tenacibaculum and Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in N. semiplicatus. (C) 2008 Elsevier Ltd. All rights reserved.
关键词Nassarius Semiplicatus Tetrodotoxin Bacteria Lc-ms Elisa 16s Rdna
学科领域Pharmacology & Pharmacy ; Toxicology
DOI10.1016/j.toxicon.2008.04.170
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000258816500007
引用统计
被引频次:65[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5396
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Hlth Inspect Agcy Lianyungang, Lianyungang 222006, Peoples R China
第一作者单位中国科学院海洋研究所
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Wang, Xiao-Jie,Yu, Ren-Cheng,Luo, Xuan,et al. Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus[J]. TOXICON,2008,52(1):55-61.
APA Wang, Xiao-Jie,Yu, Ren-Cheng,Luo, Xuan,Zhou, Ming-Jiang,&Lin, Xiang-Tian.(2008).Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus.TOXICON,52(1),55-61.
MLA Wang, Xiao-Jie,et al."Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus".TOXICON 52.1(2008):55-61.
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