Immune responses of the scallop Chlamys farreri after air exposure to different temperatures

doi:10.1016/j.jembe.2007.01.007

	Immune responses of the scallop Chlamys farreri after air exposure to different temperatures
	Chen, Mu-yan ; Yang, Hong-sheng; Delaporte, Maryse ; Zhao, San-jun ; Xing, Kun
	2007-06-29
发表期刊	JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
ISSN	0022-0981
卷号	345 期号:1 页码:52-60
文章类型	Article
摘要	The present study examined the influence of air exposure at different temperatures: a common perturbation associated with aquaculture handling practices, on immune responses in zhikong scallop Chlamys farreri. Scallops were exposed to air for 2 h, 6 h, 12 h and 24 h at 5 degrees C, 17 degrees C and 25 degrees C respectively. Thereafter, a recovery period of 24 h at 17 degrees C was applied. Haemocyte mortality, phagocytosis and reactive oxygen species (ROS) production of haemocytes, acid phosphatase (ACP) and superoxide dismutase (SOD) activity in haemocyte lysates were chosen as immumomarkers of anoxic stress. The results showed that an increase of haemocyte mortality and a decrease of phagocytosis and ACP activity were observed after 2 h of air exposure for all temperatures tested. Moreover, a significant increase of ROS production occurred following 2 h of air exposure at 25 degrees C and 24 h of air exposure at 17 degrees C. Significant differences were also observed in haemocyte mortality, percentage of phagocytic cells and ACP and SOD activity depending on the temperature of air exposure. Finally, after 24 h of recovery at 17 degrees C, percentage of phagocytic haemocytes and ACP activity did not return to initial values. ROS production was significantly higher than before the recovery period and initial values for scallops subjected to air exposure at 5 degrees C. In our study, scallops showed a relative low anoxia tolerance under a high temperature. All the scallops air exposed to 25 degrees C died after the 6 h sampling. In conclusion, air exposure associated to aquaculture practices was demonstrated to strongly affect functional immune activities of scallop haemocytes, and high temperature air exposure caused reduced survival of scallops. (c) 2007 Elsevier B.V. All rights reserved.; The present study examined the influence of air exposure at different temperatures: a common perturbation associated with aquaculture handling practices, on immune responses in zhikong scallop Chlamys farreri. Scallops were exposed to air for 2 h, 6 h, 12 h and 24 h at 5 degrees C, 17 degrees C and 25 degrees C respectively. Thereafter, a recovery period of 24 h at 17 degrees C was applied. Haemocyte mortality, phagocytosis and reactive oxygen species (ROS) production of haemocytes, acid phosphatase (ACP) and superoxide dismutase (SOD) activity in haemocyte lysates were chosen as immumomarkers of anoxic stress. The results showed that an increase of haemocyte mortality and a decrease of phagocytosis and ACP activity were observed after 2 h of air exposure for all temperatures tested. Moreover, a significant increase of ROS production occurred following 2 h of air exposure at 25 degrees C and 24 h of air exposure at 17 degrees C. Significant differences were also observed in haemocyte mortality, percentage of phagocytic cells and ACP and SOD activity depending on the temperature of air exposure. Finally, after 24 h of recovery at 17 degrees C, percentage of phagocytic haemocytes and ACP activity did not return to initial values. ROS production was significantly higher than before the recovery period and initial values for scallops subjected to air exposure at 5 degrees C. In our study, scallops showed a relative low anoxia tolerance under a high temperature. All the scallops air exposed to 25 degrees C died after the 6 h sampling. In conclusion, air exposure associated to aquaculture practices was demonstrated to strongly affect functional immune activities of scallop haemocytes, and high temperature air exposure caused reduced survival of scallops. (c) 2007 Elsevier B.V. All rights reserved.
关键词	Air Exposure Chlamys Farreri Immune Responses Survival Time Temperature
学科领域	Ecology ; Marine & Freshwater Biology
DOI	10.1016/j.jembe.2007.01.007
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000246096800005
引用统计	被引频次：90[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5322
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 3.Atlantic Vet Coll, Dept Pathol & Microbiol, Charlottetown, PE C1E 4P3, Canada
推荐引用方式 GB/T 7714	Chen, Mu-yan,Yang, Hong-sheng,Delaporte, Maryse,et al. Immune responses of the scallop Chlamys farreri after air exposure to different temperatures[J]. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,2007,345(1):52-60.
APA	Chen, Mu-yan,Yang, Hong-sheng,Delaporte, Maryse,Zhao, San-jun,&Xing, Kun.(2007).Immune responses of the scallop Chlamys farreri after air exposure to different temperatures.JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,345(1),52-60.
MLA	Chen, Mu-yan,et al."Immune responses of the scallop Chlamys farreri after air exposure to different temperatures".JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 345.1(2007):52-60.

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