Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize | |
| Sun, Feng1; Wang, Xiaomin2; Bonnard, Geraldine3; Shen, Yun4; Xiu, Zhihui4; Li, Xiaojie4; Gao, Dahai5; Zhang, Zhonghang4; Tan, Bao-Cai1 | |
| 2015-10-01 | |
| 发表期刊 | PLANT JOURNAL
 |
| 卷号 | 84期号:2页码:283-295 |
| 文章类型 | Article |
| 摘要 | RNA editing, converting cytidines (C) to uridines (U) at specific sites in the transcripts of mitochondria and plastids, plays a critical role in organelle gene expression in land plants. Recently pentatricopeptide repeat (PPR) proteins were identified as site-specific recognition factors for RNA editing. In this study, we characterized an empty pericarp7 mutant (emp7) in Zea mays (maize), which confers an embryo-lethal phenotype. In emp7 mutants, mitochondrial functions are seriously perturbed, resulting in a strikingly reduced respiration rate. Emp7 encodes an E-subgroup PPR protein that is localized exclusively in the mitochondrion. Null mutation of Emp7 abolishes the CU editing of ccmF(N) transcript solely at position 1553. CcmF(N) is coding for a subunit of heme lyase complex in the cytochromec maturation pathway. The resulting PheSer substitution in CcmF(N) leads to the loss of CcmF(N) protein and a strikingly reduced c-type cytochrome. Consequently, the mitochondrial cytochrome-linked respiratory chain is impaired as a result of the disassembly of complexIII in the emp7 mutant. These results indicate that the PPR-E subgroup protein EMP7 is required for CU editing of ccmF(N)-1553 at a position essential for cytochromec maturation and mitochondrial oxidative phosphorylation, and hence is essential to embryo and endosperm development in maize. |
| 关键词 | Maize Empty Pericarp7 Cytochromec Maturation Mitochondrion Seed Development |
| DOI | 10.1111/tpj.12993 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000362692000003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/52152 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Peoples R China 3.Associe Univ Strasbourg, Inst Biol Mol Plantes CNRS, F-67084 Strasbourg, France 4.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Feng,Wang, Xiaomin,Bonnard, Geraldine,et al. Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize[J]. PLANT JOURNAL,2015,84(2):283-295. |
| APA | Sun, Feng.,Wang, Xiaomin.,Bonnard, Geraldine.,Shen, Yun.,Xiu, Zhihui.,...&Tan, Bao-Cai.(2015).Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize.PLANT JOURNAL,84(2),283-295. |
| MLA | Sun, Feng,et al."Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize".PLANT JOURNAL 84.2(2015):283-295. |
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| Empty pericarp7 enco（1610KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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