CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules

doi:10.1016/j.dci.2015.08.002

IOCAS-IR > 实验海洋生物学重点实验室

	CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules
	Xin, Lusheng1,3 ; Zhang, Huan 1; Zhang, Ran 4; Li, Hui1,3 ; Wang, Weilin1,3 ; Wang, Lingling 1; Wang, Hao 1; Qiu, Limei 1; Song, Linsheng 1,2
	2015-12-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	53 期号:2 页码:339-348
文章类型	Article
摘要	Interleulcin 17 (IL17) is a proinflammatory cytokine that plays an important role in immune response. Recently, five novel IL17 homologs have been identified by screening and analyzing the genome of pacific oyster Crassostrea gigas. In the present study, the functions of CgIL17-5 were investigated by examining the distribution of its mRNA and protein, ligands binding and modulation in immune response. The mRNA expression levels of CgIL17-5 in hemocytes of oysters post twice challenges of Vibrio splendidus were all significantly up-regulated (P < 0.01), while the secondary pathogen infection attenuated the expression level of CgIL17-5 mRNA compared with the primary challenge. CgIL17-5 was found to be located on oyster hemocyte membranes through fluorescence confocal assay. The luciferase reporter assays showed that CgIL17-5 could activate the transfactors NF-kappa B, CREB and ATF-1, and involve in their signal pathways in HEK293T cells. Meanwhile, CgIL17-5 could augment the IL6 synthesis in HuVEC cells, playing the similar roles as human IL17 in inflammatory response. Additionally, the recombinant CgIL175 (rCgIL17-5) could directly bind peptidoglycan (PGN), lipopolysaccharide (LPS), poly (I:C) and beta-1,3-glucan, with the highest affinity to PGN, and significantly inhibit the growth of Micrococcus luteus and Escherichia coli. All the results collectively suggested that CgIL17-5, as an ancient inflammatory cytokine, could not only activate signal transduction for the release of other cytokines, but also mediate the clearance of extracellular bacteria in oysters. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Crassostrea Gigas Interleukin 17 Inflammation Pathogen-associated Molecular Pattern Recognition Bacteriostasis
DOI	10.1016/j.dci.2015.08.002
收录类别	SCI
语种	英语
WOS记录号	WOS:000362138500007
引用统计	被引频次：54[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/51033
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Xin, Lusheng,Zhang, Huan,Zhang, Ran,et al. CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(2):339-348.
APA	Xin, Lusheng.,Zhang, Huan.,Zhang, Ran.,Li, Hui.,Wang, Weilin.,...&Song, Linsheng.(2015).CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,53(2),339-348.
MLA	Xin, Lusheng,et al."CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 53.2(2015):339-348.

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