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Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus
Li, Meng1,2; Li, Caiwen1; Wang, Jinfeng1,2; Song, Shuqun1
2015-10-01
Source PublicationMOLECULAR IMMUNOLOGY
Volume67Issue:2Pages:388-397
SubtypeArticle
AbstractTolls/Toll-like receptors (TLRs) are important cell-surface receptors serving as pattern recognition receptors (PRRs) in the Tolls/TLRs signaling pathway of innate immune responses. In the present study, we isolated and characterized a novel Toll gene (PtToll) from Portunus trituberculatus, and further investigated its expression in various tissues of crab hosts challenged with the parasitic dinoflagellate Hematodinium. The full-length cDNA of PtToll was 3745 bp, with a 3012 bp open reading frame (ORF) encoding 1003 amino acids. Conserved domains consist of 15 tandem leucine-rich repeats (LRRs), a single-pass transmembrane segment (TM) and a cytoplasmic Toll/interleukin-1R (TIR) domain. The PtToll protein shared high similarity to other crustacean Tolls and was clustered with the crustacean Tolls in the phylogenetic tree. The PtToll gene was constitutively expressed in various tissues of P. trituberculatus, with the highest expression in hemocytes. After being challenged with the parasite, the transcripts of PtToll reacted immediately with significant alterations in all the tested tissues, and decreased consistently in most of the detected tissues (e.g., hemocytes, gill, heart, and muscle) within 24 h. Then the transcripts of PtToll were significantly up-regulated in hemocytes and heart at 48 h, and in hepatopancreas at 48 and 96 h post the parasitic challenge. By 192 h post challenge, the transcriptional level of PtToll indicated a significant suppression or a decreasing trend. The fluctuations of PtToll gene expression suggested that PtToll was closely associated with intrusion of the Hematodiniurn parasites, and may possess a vital and systematic function in the innate immunity of P. trituberculatus against the parasitic infection. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordCrustacean Toll Receptor Transcript Expression Innate Immunity Parasite
DOI10.1016/j.molimm.2015.06.028
Indexed BySCI
Language英语
WOS IDWOS:000361922400023
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/50937
Collection海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Meng,Li, Caiwen,Wang, Jinfeng,et al. Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus[J]. MOLECULAR IMMUNOLOGY,2015,67(2):388-397.
APA Li, Meng,Li, Caiwen,Wang, Jinfeng,&Song, Shuqun.(2015).Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus.MOLECULAR IMMUNOLOGY,67(2),388-397.
MLA Li, Meng,et al."Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus".MOLECULAR IMMUNOLOGY 67.2(2015):388-397.
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