Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia | |
Wang, Xiaofei1,2,3; Liu, Baozhong1 ; Liu, Fengsong2; Huan, Pin1
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| 2015-10-01 | |
| Source Publication | ZOOLOGICAL SCIENCE
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| Volume | 32Issue:5Pages:419-426 |
| Subtype | Article |
| Abstract | calaxin is a newly identified calcium sensor gene that modulates the movement of flagella (and possibly cilia). It was first identified from the ascidian Ciona intestinalis, and its orthologs have been observed in a wide range of animals and choanoflagellates. However, no calaxin-ortholog in a Lophotrochozoa species has been reported so far. This leaves open the question of whether the modulation of ciliary motility by calaxin is conserved among animals. We report a calaxin gene from the Pacific oyster Crassostrea gigas. This gene, termed cgi-calaxin, possesses three conserved EF-hand motifs as its orthologs from chordates. A phylogenetic analysis confirmed its orthology. Expression analysis revealed high expression in typical ciliated tissues such as gill and hepatopancreas. The spatiotemporal expression of the gene during early development was investigated using whole mount in situ hybridization. The results revealed that cgi-calaxin mRNA was aggregated in ciliated tissues of early larvae such as prototroch and velum. Immunofluorescence experiment further certified the ciliary localization of Cgi-Calaxin protein in D-veligers. We prepared the recombinant protein of cgi-calaxin and proved it had the capacity to bind to calcium. These results support the conserved roles of cgi-calaxin as a calcium sensor in ciliated cells and enrich the current knowledge on regulatory mechanisms of molluscan cilia. Moreover, we found the gene to be expressed in poorly ciliated tissues, such as adductor muscle, indicating possible roles in non-ciliated cells, which merits further investigation. |
| Keyword | Bivalve Calaxin Cilia Development Larva |
| DOI | 10.2108/zs150009 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000361955400001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/50936 |
| Collection | 实验海洋生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Hebei Univ, Baoding, Peoples R China 3.Chinese Peoples Liberat Army Gen Hosp, Dept Clin Lab, Beijing, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, Xiaofei,Liu, Baozhong,Liu, Fengsong,et al. A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia[J]. ZOOLOGICAL SCIENCE,2015,32(5):419-426. |
| APA | Wang, Xiaofei,Liu, Baozhong,Liu, Fengsong,&Huan, Pin.(2015).A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia.ZOOLOGICAL SCIENCE,32(5),419-426. |
| MLA | Wang, Xiaofei,et al."A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia".ZOOLOGICAL SCIENCE 32.5(2015):419-426. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| A calaxin Gene in th（2221KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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