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Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar
Wang, Xueyan1; Duan, Delin2; Xu, Jiachao1; Gao, Xin1; Fu, Xiaoting1
2015-10-01
Source PublicationJOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume42Issue:10Pages:1353-1362
SubtypeArticle
AbstractA bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pNPS) and agar was isolated from the coast area of Qingdao, China. It was identified as Marinomonas based on its 16S rRNA gene sequence and named as Marinomonas sp. FW-1. An arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. The enzyme was composed of a single polypeptide chain with the molecular mass of 33 kDa estimated using SDS-PAGE. The optimal pH and temperature of arylsulfatase were pH 9.0 and 45, respectively. Arylsulfatase was stable over pH 8-11 and at temperature below 55 A degrees C. The K (m) and V (max) of this enzyme for the hydrolysis of pNPS were determined to be 13.73 and 270.27 mu M/min, respectively. The desulfation ratio against agar from red seaweed Gelidium amansii and Gracilaria lemaneiformis were 86.11 and 89.61 %, respectively. There was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated G. amansii agar and that of the commercial agarose. Therefore, this novel alkaline arylsulfatase might have a great potential for application in enzymatic conversion of agar to agarose.
KeywordArylsulfatase Desulfation Agarose Purification Characterization
DOI10.1007/s10295-015-1625-6
Indexed BySCI
Language英语
WOS IDWOS:000361650000006
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/50884
Collection实验海洋生物学重点实验室
Affiliation1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xueyan,Duan, Delin,Xu, Jiachao,et al. Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(10):1353-1362.
APA Wang, Xueyan,Duan, Delin,Xu, Jiachao,Gao, Xin,&Fu, Xiaoting.(2015).Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(10),1353-1362.
MLA Wang, Xueyan,et al."Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.10(2015):1353-1362.
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