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Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar
Wang, Xueyan1; Duan, Delin2; Xu, Jiachao1; Gao, Xin1; Fu, Xiaoting1
2015-10-01
发表期刊JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷号42期号:10页码:1353-1362
文章类型Article
摘要A bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pNPS) and agar was isolated from the coast area of Qingdao, China. It was identified as Marinomonas based on its 16S rRNA gene sequence and named as Marinomonas sp. FW-1. An arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. The enzyme was composed of a single polypeptide chain with the molecular mass of 33 kDa estimated using SDS-PAGE. The optimal pH and temperature of arylsulfatase were pH 9.0 and 45, respectively. Arylsulfatase was stable over pH 8-11 and at temperature below 55 A degrees C. The K (m) and V (max) of this enzyme for the hydrolysis of pNPS were determined to be 13.73 and 270.27 mu M/min, respectively. The desulfation ratio against agar from red seaweed Gelidium amansii and Gracilaria lemaneiformis were 86.11 and 89.61 %, respectively. There was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated G. amansii agar and that of the commercial agarose. Therefore, this novel alkaline arylsulfatase might have a great potential for application in enzymatic conversion of agar to agarose.
关键词Arylsulfatase Desulfation Agarose Purification Characterization
DOI10.1007/s10295-015-1625-6
收录类别SCI
语种英语
WOS记录号WOS:000361650000006
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/50884
专题实验海洋生物学重点实验室
作者单位1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Wang, Xueyan,Duan, Delin,Xu, Jiachao,et al. Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(10):1353-1362.
APA Wang, Xueyan,Duan, Delin,Xu, Jiachao,Gao, Xin,&Fu, Xiaoting.(2015).Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(10),1353-1362.
MLA Wang, Xueyan,et al."Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.10(2015):1353-1362.
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