Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus | |
Li, Meng1,2 ; Wang, Jinfeng1,2; Song, Shuqun1; Li, Caiwen1
| |
| 2015-09-01 | |
| 发表期刊 | JOURNAL OF INVERTEBRATE PATHOLOGY
 |
| 卷号 | 130期号:2015页码:28-36 |
| 文章类型 | Article |
| 摘要 | The swimming crab Portunus trituberculatus supports a large proportion of crab aquaculture in China. In the last decade, the sustainable culture of this crab was threatened by the parasitic dinoflagellate Hematodinuim, resulting in massive mortality (up to 95%) in severely impacted culture ponds. Previous studies of Hematodinium were mainly focused on histology, molecular characterization, epizootiology, etc., with limited studies conducted to explore this specific host-parasite interaction. Thus, to give a primary insight into the anti-parasitic immune response at the critical stage of infection, the expression levels of 8 immune-related genes together with enzyme activities of phenoloxidase (PO), alkaline phosphatase (AMP), and acid phosphatase (ACP), were evaluated in hepatopancreas during 3-192 h (h) post inoculation. Hematoxylin-eosin (H&E) staining showed noticeable pathological changes in hepatopancreas. The enzyme activities of PO, AKP, and ACP were significantly induced after inoculation. The changes of the prophenoloxidase (proPO) transcripts and the constantly enhanced PO activity reflected the critical function of the proPO system in resisting against the parasites. The decreased expression levels of LGBP and PPAF implied an immunosuppressive mechanism of the parasites against the host proPO system. And the significant variations in transcriptional levels of two important proteinase inhibitors (serpin, alpha 2m) and three P. trituberculatus clip-domain serine proteinases (PTcSPs) suggested that the parasites could affect proteinase cascade reactions associated with immune response by destroying the balance between serine proteinases and the inhibitors. Moreover, the results indicated that the hepatopancreas of P. trituberculatus was significantly affected by invasion of the parasite, and hepatopancreas played important roles in the crustacean innate immunity against the parasitic infection. (C) 2015 Elsevier Inc. All rights reserved. |
| 关键词 | Crustacean Parasite Histopathology Enzyme Activity Transcription Innate Immunity |
| DOI | 10.1016/j.jip.2015.06.005 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361258800005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/50879 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Meng,Wang, Jinfeng,Song, Shuqun,et al. Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2015,130(2015):28-36. |
| APA | Li, Meng,Wang, Jinfeng,Song, Shuqun,&Li, Caiwen.(2015).Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus.JOURNAL OF INVERTEBRATE PATHOLOGY,130(2015),28-36. |
| MLA | Li, Meng,et al."Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus".JOURNAL OF INVERTEBRATE PATHOLOGY 130.2015(2015):28-36. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Early transcriptiona（2229KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论