Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri | |
Wang, Mengqiang1 ; Wang, Lingling1; Guo, Ying1; Sun, Rui1; Yue, Feng1; Yi, Qilin1; Song, Linsheng1,2
| |
| 2015-10-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 卷号 | 52期号:2页码:192-201 |
| 文章类型 | Article |
| 摘要 | Toll-like receptors (TLRs) are among the most studied pattern recognition receptors (PRRs) playing essential roles in innate immune defenses. In the present study, the basic features of CfTLR in mollusk Zhikong scallop Chlamys jarred. including sequence homology, tissue distribution, subcellular localization and ligands spectrum, were investigated to elucidate its pattern recognition. The elements of extracellular domains (ECD) in CfTLR displayed high homology to the corresponding parts of the ECDs in TLRs from Homo sapiens. CfTLR protein was detected in hemocytes, mantle, gills, hepatopancreas, kidney and gonad of the scallops, and it was localized in both the plasma membranes and the lysosomes in HEK293T cells. MLR could activate NF kappa B in response to multiple HsTLR ligands including Pam3CSK4, glucan (GLU), peptidoglycan (PGN), polyriboinosinic:polyribocytidylic acid (poly I:C), Imiquimod and three types of CpG. Additionally, the scallop serum could enhance the induction of NF kappa B in the CfTLR expressing cells elicited by most PAMPs, including GLU, PGN, Imiquimod and four types of CpG. It could be concluded that this primitive mollusk TLR shared a hybrid function in pattern recognition and could recognize broader ligands than mammalian TLRs, and its mosaic capability of pathogen associated molecular pattern (PAMP) recognition might be based on the basic features of its structure, ligand properties and the assistance of some components in scallop serum. (C) 2015 Elsevier Ltd. All rights reserved. |
| 关键词 | Chlamys Farreri Innate Immunity Pattern Recognition Toll-like Receptor |
| DOI | 10.1016/j.dci.2015.05.011 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360592200010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/50762 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Mengqiang,Wang, Lingling,Guo, Ying,et al. The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,52(2):192-201. |
| APA | Wang, Mengqiang.,Wang, Lingling.,Guo, Ying.,Sun, Rui.,Yue, Feng.,...&Song, Linsheng.(2015).The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,52(2),192-201. |
| MLA | Wang, Mengqiang,et al."The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 52.2(2015):192-201. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| The broad pattern re（2106KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论