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Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis
Yang, Hui1,2; Li, Shihao1,3; Li, Fuhua1,3; Lv, Xinjia1,2; Xiang, Jianhai1
2015-11-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume53Issue:1Pages:47-54
SubtypeArticle
AbstractAntimicrobial peptides (AMPs) have a great potential to be used as a substitute for antibiotics since AMPs don't lead to bacteria's drug resistance. Anti-lipopolysaccharide factors (ALFs) are one type of AMPs and exist in crustaceans. In the present study, we produced a recombinant protein (rFcALF5) of an ALP isoform (FcALF5) from Chinese shrimp Fenneropenaeus chinensis through a prokaryotic expression system. The rFcALF5 exhibited varied antibacterial activities against different bacteria. Besides its antibacterial activities, it could also inhibit the infection of white spot syndrome virus (WSSV) to shrimp after preincubation with this virus. In order to learn the antiviral mechanism on how rFcALF5 influences WSSV infection, the interaction between the total proteins of WSSV and rFcALF5 was analyzed and the data showed that rFcALF5 had direct interaction with the envelope protein VP24 of WSSV. The LPS binding domain (LBD) of FcALF5 also showed direct interaction with VP24 of WSSV. Therefore we inferred that the antiviral activity of FcALF5 might be achieved through the binding of its LBD to VP24 of WSSV. These findings provided more information to develop new strategies for the control of shrimp disease in aquaculture. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordAnti-lipopolysaccharide Factors Antibacterial Antiviral Vp24 Wssv
DOI10.1016/j.dci.2015.06.015
Indexed BySCI
Language英语
WOS IDWOS:000360772900006
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/50421
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Hui,Li, Shihao,Li, Fuhua,et al. Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(1):47-54.
APA Yang, Hui,Li, Shihao,Li, Fuhua,Lv, Xinjia,&Xiang, Jianhai.(2015).Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,53(1),47-54.
MLA Yang, Hui,et al."Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 53.1(2015):47-54.
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