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High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis
Cui, Z.1,2; Hui, M.1; Liu, Y.1; Song, C.1,3; Li, X.1,3; Li, Y.1; Liu, L.1; Shi, G.1,3; Wang, S.4; Li, F.1; Zhang, X.1; Liu, C.1; Xiang, J.1; Chu, K. H.5
2015-09-01
Source PublicationHEREDITY
Volume115Issue:3Pages:206-215
SubtypeArticle
AbstractThe sex determination system in crabs is believed to be XY-XX from karyotypy, but centromeres could not be identified in some chromosomes and their morphology is not completely clear. Using quantitative trait locus mapping of the gender phenotype, we revealed a ZW-ZZ sex determination system in Eriocheir sinensis and presented a high-density linkage map covering similar to 98.5% of the genome, with 73 linkage groups corresponding to the haploid chromosome number. All sex-linked markers in the family we used were located on a single linkage group, LG60, and sex linkage was confirmed by genome-wide association studies (GWAS). Forty-six markers detected by GWAS were heterozygous and segregated only in the female parent. The female LG60 was thus the putative W chromosome, with the homologous male LG60 as the Z chromosome. The putative Z and W sex chromosomes were identical in size and carried many homologous loci. Sex ratio (5: 1) skewing towards females in induced triploids using unrelated animals also supported a ZW-ZZ system. Transcriptome data were used to search for candidate sex-determining loci, but only one LG60 gene was identified as an ankyrin-2 gene. Double sex- and mab3-related transcription factor 1 (Dmrt1), a Z-linked gene in birds, was located on a putative autosome. With complete genome sequencing and transcriptomic data, more genes on putative sex chromosomes will be characterised, thus leading towards a comprehensive understanding of the sex determination and differentiation mechanisms of E. sinensis, and decapod crustaceans in general.
DOI10.1038/hdy.2015.26
Indexed BySCI
Language英语
WOS IDWOS:000359458400004
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49947
Collection实验海洋生物学重点实验室
海洋环流与波动重点实验室
海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Qingdao, Peoples R China
5.Chinese Univ Hong Kong, Sch Life Sci, Simon FS Li Marine Sci Lab, Hong Kong, Hong Kong, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;  Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Cui, Z.,Hui, M.,Liu, Y.,et al. High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis[J]. HEREDITY,2015,115(3):206-215.
APA Cui, Z..,Hui, M..,Liu, Y..,Song, C..,Li, X..,...&Chu, K. H..(2015).High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis.HEREDITY,115(3),206-215.
MLA Cui, Z.,et al."High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis".HEREDITY 115.3(2015):206-215.
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