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Screening for hydrogen-producing strains of green microalgae in phosphorus or sulphur deprived medium under nitrogen limitation
Pongpadung, Piyawat1,2; Liu, Jianguo3; Yokthongwattana, Kittisak4,5; Techapinyawat, Sombun2,6; Juntawong, Niran1,2,6
2015-04-01
Source PublicationSCIENCEASIA
Volume41Issue:2Pages:97-107
SubtypeArticle
AbstractHydrogen gas, one of the best candidates for clean and renewable energy, can be produced by microalgae that can use solar energy to cause photolysis of water. This study screened H-2-producing indigenous green microalgae under sulphur (S-) deprivation, simultaneous nitrogen (N-) limitation and S-deprivation, or simultaneous N-limitation and phosphorus (P-) deprivation. Sequences of 18S rDNA and ITS in conjunction with morphological characteristics were used to identify the algae. We report that Chlorella lewinii, Micractinium sp., Coelastrella sp., and Monoraphidium sp. have the ability to produce H-2. The increase in H-2 photoproduction when N is limited seems to be a universal phenomenon in most tested strains of Chlorella, in all strains of Chlamydomonas, but in no strain of Coelastrella, Micractinium, or Scenedesmus. Chlorella sorokiniana KU204 produced H-2 (46 ml/l) under S-deprivation. This strain exhibited the highest H-2-producing ability (1.30 ml l(-1) h(-1)) and accumulated up to 90 ml/l under simultaneous N-limitation and S-deprivation. Interestingly, C. sorokiniana KU204 could also produce H-2 under simultaneous N-limitation and P-starvation (69 ml/l). The induction time to reach an anoxic state by most tested strains of Chlorella, but not Chlamydomonas, was shorter under simultaneous N-limitation and S-deprivation than under S-deprivation. In addition, those strains of Chlorella exhibited high H-2 photoproduction under simultaneous N-limitation and S-deprivation. A few Chlorella strains were unable to reach an anoxic state during the experiment. However, such regularity is not found in Chlamydomonas. The results indicate that the mechanism of H-2 photoproduction in Chlorella may differ from that in Chlamydomonas.
KeywordHydrogen Production Green Algae Sulphur Deprivation Phosphorus Deprivation
DOI10.2306/scienceasia1513-1874.2015.41.097
Indexed BySCI
Language英语
WOS IDWOS:000359364700004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49780
Collection海洋生物技术研发中心
Affiliation1.Kasetsart Univ, Fac Sci, Res Ctr Photosynthet Pigments & Secondary Metab, Bangkok 10900, Thailand
2.Kasetsart Univ, Biosci Program, Fac Sci, Bangkok 10900, Thailand
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
4.Mahidol Univ, Fac Sci, Dept Biochem, Bangkok 10400, Thailand
5.Mahidol Univ, Fac Sci, Ctr Excellence Prot Struct & Funct, Bangkok 10400, Thailand
6.Kasetsart Univ, Fac Sci, Dept Bot, Bangkok 10900, Thailand
Recommended Citation
GB/T 7714
Pongpadung, Piyawat,Liu, Jianguo,Yokthongwattana, Kittisak,et al. Screening for hydrogen-producing strains of green microalgae in phosphorus or sulphur deprived medium under nitrogen limitation[J]. SCIENCEASIA,2015,41(2):97-107.
APA Pongpadung, Piyawat,Liu, Jianguo,Yokthongwattana, Kittisak,Techapinyawat, Sombun,&Juntawong, Niran.(2015).Screening for hydrogen-producing strains of green microalgae in phosphorus or sulphur deprived medium under nitrogen limitation.SCIENCEASIA,41(2),97-107.
MLA Pongpadung, Piyawat,et al."Screening for hydrogen-producing strains of green microalgae in phosphorus or sulphur deprived medium under nitrogen limitation".SCIENCEASIA 41.2(2015):97-107.
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