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Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation
Zhang, Zhendong1; Mo, Zhaolan2; Chen, Shiyong2; Zou, YuXia2; Zhang, Peijun2
2015-07-01
Source PublicationJOURNAL OF ENVIRONMENTAL BIOLOGY
Volume36Issue:2015Pages:807-811
SubtypeArticle
AbstractVibrio anguillarum strain M3 was isolated from cultured diseased flounder Paralichthys olivaceus. In order to determine whether chromosomal angR gene plays an important role in infecting flounder host, part fragment of a virulence regulatory gene angR from V anguillarum M3 genome was cloned and ligated with suicide plasmids pNQ705 in the present study. The conserved fragment of angR gene was amplified from M3 genome and inserted into suicide plasmid pNQ705, a kind of conditional replicon. The recombinant plasmid was transferred into Vibrio anguillarum M3 strain genomic DNA through bacterial conjugation and homologous recombination. A mutant V anguillarum strain with angR gene mutation was constructed and screened successfully using TCBS medium containing chloramphenicol. PCR identification and sequencing showed that the recombinant plasmid inserted into Vibrio anguillarum genome DNA, as expected design, resulted in insertional inactivation of angR gene. Results of artificial infection experiment showed that virulence of mutant Vibrio anguillarum strain reduced dramatically as compared with the wild strain M3.
KeywordAngr Gene Iron Uptake System Insertional Inactivation Vibrio Anguillarum
Indexed BySCI
Language英语
WOS IDWOS:000358754200014
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49673
Collection实验海洋生物学重点实验室
Affiliation1.Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhendong,Mo, Zhaolan,Chen, Shiyong,et al. Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation[J]. JOURNAL OF ENVIRONMENTAL BIOLOGY,2015,36(2015):807-811.
APA Zhang, Zhendong,Mo, Zhaolan,Chen, Shiyong,Zou, YuXia,&Zhang, Peijun.(2015).Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation.JOURNAL OF ENVIRONMENTAL BIOLOGY,36(2015),807-811.
MLA Zhang, Zhendong,et al."Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation".JOURNAL OF ENVIRONMENTAL BIOLOGY 36.2015(2015):807-811.
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