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Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis
Zhang, Bao Yu1; Hou, Zhao Jun2; Wang, Guang Ce1; Peng, Guang1
2015-08-01
Source PublicationBOTANICA MARINA
Volume58Issue:4Pages:285-293
SubtypeArticle
AbstractMalate dehydrogenase (MDH) is an enzyme that catalyzes the interconversion of malate and oxaloacetate substrates and is widely distributed from prokaryotes to eukaryotes. It plays crucial roles in many important metabolic pathways and includes different isoforms based on coenzyme specificity and cellular localization. To study MDH in rhodophytes, we obtained a full-length cDNA clone (here designated PhMDH) encoding an NAD-malate dehydrogenase in the marine red alga Pyropia haitanensis. The nucleotide sequence of PhMDH was 1521 bp, including an open reading frame (ORF) of 984 bp. The amino acid sequence showed 73% identity with other MDHs in proteobacteria. Two MDH-like domains were detected in the 5-145 and 156-320 regions. Real-time fluorescent quantitative PCR (qPCR) was used to examine mRNA expression levels during the gametophyte and sporophyte phases. The transcription of PhMDH in the gametophyte was barely detectable, whereas PhMDH in the sporophyte showed a much higher expression level. The activity of PhMDH in the filamentous sporophyte was approximately double that of the leafy gametophyte. Considering these results, we suggest that PhMDH may be localized in the cytosol and play a role in carbon fixation in the sporophyte stage.
KeywordActivity Nad-malate Dehydrogenase Qpcr
DOI10.1515/bot-2014-0052
Indexed BySCI
Language英语
WOS IDWOS:000358409200005
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49669
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Tianjin Univ Sci & Technol, Coll Comp Sci & Informat Engn, Tianjin 300457, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
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Zhang, Bao Yu,Hou, Zhao Jun,Wang, Guang Ce,et al. Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis[J]. BOTANICA MARINA,2015,58(4):285-293.
APA Zhang, Bao Yu,Hou, Zhao Jun,Wang, Guang Ce,&Peng, Guang.(2015).Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis.BOTANICA MARINA,58(4),285-293.
MLA Zhang, Bao Yu,et al."Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis".BOTANICA MARINA 58.4(2015):285-293.
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