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Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis
Zhang, Bao Yu1; Hou, Zhao Jun2; Wang, Guang Ce1; Peng, Guang1
2015-08-01
发表期刊BOTANICA MARINA
卷号58期号:4页码:285-293
文章类型Article
摘要Malate dehydrogenase (MDH) is an enzyme that catalyzes the interconversion of malate and oxaloacetate substrates and is widely distributed from prokaryotes to eukaryotes. It plays crucial roles in many important metabolic pathways and includes different isoforms based on coenzyme specificity and cellular localization. To study MDH in rhodophytes, we obtained a full-length cDNA clone (here designated PhMDH) encoding an NAD-malate dehydrogenase in the marine red alga Pyropia haitanensis. The nucleotide sequence of PhMDH was 1521 bp, including an open reading frame (ORF) of 984 bp. The amino acid sequence showed 73% identity with other MDHs in proteobacteria. Two MDH-like domains were detected in the 5-145 and 156-320 regions. Real-time fluorescent quantitative PCR (qPCR) was used to examine mRNA expression levels during the gametophyte and sporophyte phases. The transcription of PhMDH in the gametophyte was barely detectable, whereas PhMDH in the sporophyte showed a much higher expression level. The activity of PhMDH in the filamentous sporophyte was approximately double that of the leafy gametophyte. Considering these results, we suggest that PhMDH may be localized in the cytosol and play a role in carbon fixation in the sporophyte stage.
关键词Activity Nad-malate Dehydrogenase Qpcr
DOI10.1515/bot-2014-0052
收录类别SCI
语种英语
WOS记录号WOS:000358409200005
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/49669
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Tianjin Univ Sci & Technol, Coll Comp Sci & Informat Engn, Tianjin 300457, Peoples R China
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Zhang, Bao Yu,Hou, Zhao Jun,Wang, Guang Ce,et al. Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis[J]. BOTANICA MARINA,2015,58(4):285-293.
APA Zhang, Bao Yu,Hou, Zhao Jun,Wang, Guang Ce,&Peng, Guang.(2015).Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis.BOTANICA MARINA,58(4),285-293.
MLA Zhang, Bao Yu,et al."Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis".BOTANICA MARINA 58.4(2015):285-293.
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