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Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis
Liu, Li1,2; Chi, Heng1; Sun, Li1
2015-06-29
Source PublicationDISEASES OF AQUATIC ORGANISMS
Volume115Issue:1Pages:67-80
SubtypeArticle
AbstractPseudomonas fluorescens is a Gram-negative bacterium and a common pathogen to a wide range of farmed fish. In a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of P. fluorescens (wild-type strain TSS). In the present work, we conducted comparative proteomic analysis to examine the global protein profiles of TSS and the P. fluorescens fur knockout mutant TFM. Twenty-eight differentially produced proteins were identified, which belong to different functional categories. Four of these proteins, viz. TssP (a type VI secretion protein), PspA (a serine protease), OprF (an outer membrane porin), and ClpP (the proteolytic subunit of an ATP-dependent Clp protease), were assessed for virulence participation in a model of turbot Scophthalmus maximus. The results showed that the oprF and clpP knockouts exhibited significantly reduced capacities in (1) resistance against the bactericidal effect of host serum, (2) dissemination into and colonization of host tissues, and (3) inducing host mortality. In contrast, mutation of tssP and pspA had no apparent effect on the pathogenicity of TSS. Purified recombinant OprF, when used as a subunit vaccine, induced production of specific serum antibodies in immunized fish and elicited significant protection against lethal TSS challenge. Antibody blocking of the OprF in TSS significantly impaired the ability of the bacteria to invade host tissues. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, Fur regulates the expression of diverse proteins, some of which are required for optimal infection.
KeywordPseudomonas Fluorescens Ferric Uptake Regulator Proteomic Infection Virulence Vaccine
DOI10.3354/dao02874
Indexed BySCI
Language英语
WOS IDWOS:000357598200008
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49204
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Li,Chi, Heng,Sun, Li. Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis[J]. DISEASES OF AQUATIC ORGANISMS,2015,115(1):67-80.
APA Liu, Li,Chi, Heng,&Sun, Li.(2015).Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis.DISEASES OF AQUATIC ORGANISMS,115(1),67-80.
MLA Liu, Li,et al."Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis".DISEASES OF AQUATIC ORGANISMS 115.1(2015):67-80.
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