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CD83 is required for the induction of protective immunity by a DNA vaccine in a teleost model
Li, Mo-fei1,4; Li, Yong-xin2; Sun, Li1,3
2015-07-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号51期号:1页码:141-147
文章类型Article
摘要In mammals, CD83 is a surface marker on mature dendritic cells and vital to lymphocyte activation. In teleost, studies on the function of CD83 are very limited. In this study, we examined the potential involvement of turbot (Scophthalmus maximus) CD83, SmCD83, in vaccine-induced immunity. For this purpose, turbot were immunized with pORF75, a DNA vaccine against megalocytivirus, in the presence or absence of pSmCD83, a plasmid that constitutively expresses SmCD83. Immune response and protection analysis showed that the presence of pSmCD83 significantly (i) enhanced the activation of head kidney macrophages (HKM) and immune gene expression, (ii) inhibited viral replication in fish tissues following megalocytivirus challenge and increased the survival of the vaccinated fish, and (iii) stimulated production of specific serum antibody and the cytotoxicity of peripheral blood leukocytes. To further examine the effect of SmCD83, pORF75 was administered into turbot in which SmCD83 was knocked down. Subsequent analysis showed that in fish with SmCD83 knockdown, vaccine-induced HKM activation and antibody production were severely reduced, and, consistently, the protectivity of pORF75 was drastically decreased. Taken together, these results indicate for the first time that teleost CD83 is required for the induction of protective immune response by DNA vaccine. (C) 2015 Elsevier Ltd. All rights reserved.
关键词Cd83 Dna Vaccine Scophthalmus Maximus Immune Response Adjuvant
DOI10.1016/j.dci.2015.03.005
收录类别SCI
语种英语
WOS记录号WOS:000357906800017
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/49202
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Taishan Vocat Coll Nursing, Tai An 271000, Shandong, Peoples R China
3.Zhejiang Univ, Collaborat Innovat Ctr Deep Sea Biol, Hangzhou 310003, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Li, Mo-fei,Li, Yong-xin,Sun, Li. CD83 is required for the induction of protective immunity by a DNA vaccine in a teleost model[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):141-147.
APA Li, Mo-fei,Li, Yong-xin,&Sun, Li.(2015).CD83 is required for the induction of protective immunity by a DNA vaccine in a teleost model.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),141-147.
MLA Li, Mo-fei,et al."CD83 is required for the induction of protective immunity by a DNA vaccine in a teleost model".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):141-147.
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