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Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties
Wang, Jing-jing1,2; Sun, Li1
2015-08-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
Volume41Issue:4Pages:951-959
SubtypeArticle
AbstractFerritin is an evolutionarily conserved protein that plays a vital role in maintaining iron homeostasis. In this study, we identified a ferritin M (PoFerM) from Japanese flounder (Paralichthys olivaceus) and analyzed its biological property. PoFerM is composed of 176 amino acid residues and contains the conserved ferroxidase diiron center and the ferrihydrite nucleation center typical of M ferritins. Expression of PoFerM occurred in multiple tissues and was most abundant in blood. Bacterial infection upregulated PoFerM expression in head kidney, spleen, and liver in a time-dependent manner. Recombinant PoFerM (rPoFerM) purified from Escherichia coli exhibited iron-chelating activity and inhibited bacterial growth, whereas rPoFerMM, the mutant protein that bears alanine substitution at two conserved residues of the ferroxidase center and the ferrihydrite nucleation center, failed to do so. Oxidative protection analysis showed that rPoFerM, but not rPoFerMM, was able to alleviate the deleterious effect of H2O2-induced free radicals on plasmid DNA and primary flounder cells. Together these results indicate that PoFerM is an iron chelator with antimicrobial and antioxidative properties, all which depend on the conserved ferroxidase center and the ferrihydrite nucleation site.
KeywordAntimicrobial Antioxidative Ferritin Iron Binding Paralichthys Olivaceus
DOI10.1007/s10695-015-0060-y
Indexed BySCI
Language英语
WOS IDWOS:000357660700011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49201
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Jing-jing,Sun, Li. Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(4):951-959.
APA Wang, Jing-jing,&Sun, Li.(2015).Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties.FISH PHYSIOLOGY AND BIOCHEMISTRY,41(4),951-959.
MLA Wang, Jing-jing,et al."Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.4(2015):951-959.
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