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Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties
Wang, Jing-jing1,2; Sun, Li1
2015-08-01
发表期刊FISH PHYSIOLOGY AND BIOCHEMISTRY
卷号41期号:4页码:951-959
文章类型Article
摘要Ferritin is an evolutionarily conserved protein that plays a vital role in maintaining iron homeostasis. In this study, we identified a ferritin M (PoFerM) from Japanese flounder (Paralichthys olivaceus) and analyzed its biological property. PoFerM is composed of 176 amino acid residues and contains the conserved ferroxidase diiron center and the ferrihydrite nucleation center typical of M ferritins. Expression of PoFerM occurred in multiple tissues and was most abundant in blood. Bacterial infection upregulated PoFerM expression in head kidney, spleen, and liver in a time-dependent manner. Recombinant PoFerM (rPoFerM) purified from Escherichia coli exhibited iron-chelating activity and inhibited bacterial growth, whereas rPoFerMM, the mutant protein that bears alanine substitution at two conserved residues of the ferroxidase center and the ferrihydrite nucleation center, failed to do so. Oxidative protection analysis showed that rPoFerM, but not rPoFerMM, was able to alleviate the deleterious effect of H2O2-induced free radicals on plasmid DNA and primary flounder cells. Together these results indicate that PoFerM is an iron chelator with antimicrobial and antioxidative properties, all which depend on the conserved ferroxidase center and the ferrihydrite nucleation site.
关键词Antimicrobial Antioxidative Ferritin Iron Binding Paralichthys Olivaceus
DOI10.1007/s10695-015-0060-y
收录类别SCI
语种英语
WOS记录号WOS:000357660700011
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/49201
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Jing-jing,Sun, Li. Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(4):951-959.
APA Wang, Jing-jing,&Sun, Li.(2015).Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties.FISH PHYSIOLOGY AND BIOCHEMISTRY,41(4),951-959.
MLA Wang, Jing-jing,et al."Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.4(2015):951-959.
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