| Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense | |
Qu, Tao1; Zhang, Linlin1; Wang, Wei1; Huang, Baoyu1,2 ; Li, Yingxiang1,2; Zhu, Qihui1,2; Li, Li1; Zhang, Guofan1
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| 2015-07-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 卷号 | 51期号:1页码:74-78 |
| 文章类型 | Article |
| 摘要 | The inhibitor of apoptosis (IAP) proteins maintain a balance between cell proliferation and cell death by inhibiting caspase activity and facilitating immune responses. In this study, phylogenetic analysis revealed lineage-specific expansion and tandem duplication of IAPs in the Pacific oyster Crassostrea gigas. We then investigated a representative oyster-specific XIAP-like gene (CgIAP2) to understand how it regulates initiator caspase. Cloning of full-length CgIAP2 from oyster cDNA uncovered a deduced protein containing two BIR domains and a RING domain. Homolog comparison demonstrated that CgIAP2 clustered into the invertebrate branch. We found that CgIAP2 was likely involved in apoptosis inhibition and immune defense, based on high mRNA expression in the gills and labial palps, as well as increased mRNA expression after bacterial challenge. A yeast two-hybrid assay revealed that the BIR2 domain was necessary and sufficient to mediate interaction between CgIAP2 and Cgcaspase-2, providing direct evidence that CgIAP2 participates in apoptosis inhibition. (C) 2015 Elsevier Ltd. All rights reserved. |
| 关键词 | Crassostrea Gigas Iap Evolution Intrinsic Apoptosis Caspase Immune Response |
| DOI | 10.1016/j.dci.2015.02.011 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357906800009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/49050 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Qu, Tao,Zhang, Linlin,Wang, Wei,et al. Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):74-78. |
| APA | Qu, Tao.,Zhang, Linlin.,Wang, Wei.,Huang, Baoyu.,Li, Yingxiang.,...&Zhang, Guofan.(2015).Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),74-78. |
| MLA | Qu, Tao,et al."Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):74-78. |
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