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Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei
Yang, Hui1,2; Li, Shihao1,3; Li, Fuhua1,3; Wen, Rong1; Xiang, Jianhai1
2015-08-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume51Issue:2Pages:278-286
SubtypeArticle
AbstractSyndecan is considered to be a multifunctional protein which functions as a cell surface receptor involved in cell adhesion, migration, cytoskeleton organization and differentiation. Previous bioinformatic analysis has revealed that syndecan in shrimp might interact with white spot syndrome virus (WSSV). In the present study, we experimentally studied the function of syndecan in shrimp immunity. The syndecan from Litopenaeus vannamei (LvSDC) was cloned and analyzed. The full-length cDNA of LvSDC was 1005 bp, consisting of 59 bp 5'-UTR, 253 bp 3'-UTR, and 693 bp open reading frame encoding 230 amino acids. LvSDC consisted of an extracellular domain (ED), a transmembrane domain (TM) and a cytoplasmic domain (CD). TM and CD shared high similarities with those of syndecan proteins from other species. LvSDC was ubiquitously expressed in all tested tissues, with the highest level in Oka. After WSSV challenge, the transcription level of LvSDC in Oka was apparently up-regulated. Recombinant LvSDC protein and its rabbit polyclonal antibody were prepared for detecting the location of LvSDC in hemocytes using immunocytochemistry approach. Data showed that LvSDC mainly located at the cell membrane and the cytoplasm of hemocytes. After silencing of LvSDC with siRNA, the WSSV copy numbers and mortality of shrimp after WSSV infection were both significantly decreased. These data provide useful information for understanding the immune mechanism of shrimp to WSSV infection. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordSyndecan Wssv Infection Sirna Penaeid Shrimp
DOI10.1016/j.dci.2015.03.013
Indexed BySCI
Language英语
WOS IDWOS:000357910600007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49049
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
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Yang, Hui,Li, Shihao,Li, Fuhua,et al. Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(2):278-286.
APA Yang, Hui,Li, Shihao,Li, Fuhua,Wen, Rong,&Xiang, Jianhai.(2015).Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(2),278-286.
MLA Yang, Hui,et al."Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.2(2015):278-286.
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