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Improvement of H-2 photoproduction in Chlorella pyrenoidosa in artificial and natural seawater by addition of acetic acid and control of nutrients
He, Meilin1,2; Li, Ling1; Liu, Jianguo1; Zhang, Litao1
2015-07-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume10Issue:2015Pages:104-109
SubtypeArticle
AbstractTwo marine Chlorella strains, Chlorella pyrenoidosa 689S and C. pyrenoidosa 707S, were previously found to generate H-2 gas under sulfur (S) deprivation. In this work, their ability to generate H-2 under sulfur-replete, artificial and natural seawater conditions was further explored. C. pyrenoidosa 689S and 707S evolved 20.77 and 25.51 ml/lH(2), respectively, in artificial seawater (ASW) without any nutrient deprivation, simply by adding acetic acid. Sulfur or nitrogen (N) deprivation promoted H-2 production by C. pyrenoidosa 689S and 707S incubated in ASW, while phosphorous (P) deprivation did not improve H-2 production effectively. Changes of culture medium from ASW-S to TAP'-S induced an increase of similar to 14% and 200% in H-2 yield in C. pyrenoidosa 689S and 707S respectively. However, the Chlorella cells did not grow well in TAP' medium, which contains NH4Cl as nitrogen source, at approximately the same molar concentration as the nitrogen in ASW medium, as indicated by the low chlorophyll content in TAP'-S cultures. When natural seawater was used as the water source for culture medium instead of the ASW prepared using distilled water, the H-2 production by C. pyrenoidosa 689S and 707S was significantly altered. Both Chlorella strains failed to transit to the anaerobic phase and evolved no H-2 in L1 medium containing acetate prepared using natural seawater, implying that some unknown factor(s) in natural seawater might hinder the establishment of anaerobiosis and activation of H(2)ase. Even N and P deprivation did not induce H-2 production in C. pyrenoidosa 689S. Although C. pyrenoidosa 707S was not able to evolve H-2 under P-deprivation conditions in natural seawater medium, it was found, surprisingly, to generate comparable amounts of H-2 in N-free medium prepared using natural seawater as in N-free ASW medium, showing that the strain might be useful for biological H-2 photoproduction. (C) 2015 Elsevier B.V. All rights reserved.
KeywordH-2 Photoproduction Marine Chlorella Sulfur Deprivation Nitrogen Deprivation Natural Seawater
DOI10.1016/j.algal.2015.04.018
Indexed BySCI
Language英语
WOS IDWOS:000357989300014
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49048
Collection海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
2.Nanjing Agr Univ, Coll Resource & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
He, Meilin,Li, Ling,Liu, Jianguo,et al. Improvement of H-2 photoproduction in Chlorella pyrenoidosa in artificial and natural seawater by addition of acetic acid and control of nutrients[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2015,10(2015):104-109.
APA He, Meilin,Li, Ling,Liu, Jianguo,&Zhang, Litao.(2015).Improvement of H-2 photoproduction in Chlorella pyrenoidosa in artificial and natural seawater by addition of acetic acid and control of nutrients.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,10(2015),104-109.
MLA He, Meilin,et al."Improvement of H-2 photoproduction in Chlorella pyrenoidosa in artificial and natural seawater by addition of acetic acid and control of nutrients".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 10.2015(2015):104-109.
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