Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus

doi:10.1111/jfb.12700

IOCAS-IR > 实验海洋生物学重点实验室

	Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus
	Zheng, Y.1; Peng, L. M.1,2; You, F.1; Zou, Y. X.1; Zhang, P. J.1; Chen, S. L.3
	2015-07-01
发表期刊	JOURNAL OF FISH BIOLOGY
卷号	87 期号:1 页码:115-122
文章类型	Article
摘要	A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.
关键词	Astrocyte Brain-cell Line Epithelioid-like Karyotype Transfection
DOI	10.1111/jfb.12700
收录类别	SCI
语种	英语
WOS记录号	WOS:000357686800009
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/48988
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zheng, Y.,Peng, L. M.,You, F.,et al. Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus[J]. JOURNAL OF FISH BIOLOGY,2015,87(1):115-122.
APA	Zheng, Y.,Peng, L. M.,You, F.,Zou, Y. X.,Zhang, P. J.,&Chen, S. L..(2015).Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus.JOURNAL OF FISH BIOLOGY,87(1),115-122.
MLA	Zheng, Y.,et al."Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus".JOURNAL OF FISH BIOLOGY 87.1(2015):115-122.

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