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Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus
Zheng, Y.1; Peng, L. M.1,2; You, F.1; Zou, Y. X.1; Zhang, P. J.1; Chen, S. L.3
2015-07-01
发表期刊JOURNAL OF FISH BIOLOGY
卷号87期号:1页码:115-122
文章类型Article
摘要A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.
关键词Astrocyte Brain-cell Line Epithelioid-like Karyotype Transfection
DOI10.1111/jfb.12700
收录类别SCI
语种英语
WOS记录号WOS:000357686800009
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/48988
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao, Shandong, Peoples R China
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Zheng, Y.,Peng, L. M.,You, F.,et al. Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus[J]. JOURNAL OF FISH BIOLOGY,2015,87(1):115-122.
APA Zheng, Y.,Peng, L. M.,You, F.,Zou, Y. X.,Zhang, P. J.,&Chen, S. L..(2015).Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus.JOURNAL OF FISH BIOLOGY,87(1),115-122.
MLA Zheng, Y.,et al."Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus".JOURNAL OF FISH BIOLOGY 87.1(2015):115-122.
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