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Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus
Zheng, Y.1; Peng, L. M.1,2; You, F.1; Zou, Y. X.1; Zhang, P. J.1; Chen, S. L.3
2015-07-01
Source PublicationJOURNAL OF FISH BIOLOGY
Volume87Issue:1Pages:115-122
SubtypeArticle
AbstractA new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.
KeywordAstrocyte Brain-cell Line Epithelioid-like Karyotype Transfection
DOI10.1111/jfb.12700
Indexed BySCI
Language英语
WOS IDWOS:000357686800009
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/48988
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zheng, Y.,Peng, L. M.,You, F.,et al. Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus[J]. JOURNAL OF FISH BIOLOGY,2015,87(1):115-122.
APA Zheng, Y.,Peng, L. M.,You, F.,Zou, Y. X.,Zhang, P. J.,&Chen, S. L..(2015).Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus.JOURNAL OF FISH BIOLOGY,87(1),115-122.
MLA Zheng, Y.,et al."Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus".JOURNAL OF FISH BIOLOGY 87.1(2015):115-122.
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