Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri

doi:10.1007/s00343-009-9120-4

IOCAS-IR > 海洋地质与环境重点实验室

	Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri
	Saren Gaowa 1,2; Zhao Yonggang 3
	2009-06-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:2 页码:324-330
文章类型	Article
摘要	A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind gut and testis, hepatic caecum, gill, endostyle, and epipharyngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.; A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind gut and testis, hepatic caecum, gill, endostyle, and epipharyngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.
关键词	Amphioxus B. B.lcheri Amphi-phlp Northern Blotting In Situ Hybridization Cellular Localization
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9120-4
收录类别	SCI
语种	英语
WOS记录号	WOS:000267970300017
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3694
专题	海洋地质与环境重点实验室
作者单位	1.Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 3.China Anim Hlth & Epidemiol Ctr, Qingdao 266032, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Saren Gaowa,Zhao Yonggang. Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(2):324-330.
APA	Saren Gaowa,&Zhao Yonggang.(2009).Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(2),324-330.
MLA	Saren Gaowa,et al."Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.2(2009):324-330.

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