Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro

doi:10.1016/j.carbpol.2005.04.007

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	Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro
	Xing, RE; Liu, S ; Yu, HH; Guo, ZY ; Li, Z ; Li, PC
	2005-08-04
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	61 期号:2 页码:148-154
文章类型	Article
摘要	The influence of molecular weight and substitution degree of sulfated polysaccharides on their biological activity is considered in majority of works involving the anticoagulant or antiviral properties of these substances. Therefore, the present paper describes the effect of preparation conditions of sulfated chitosans on their molecular weight and sulfur content, such as different reaction time, acid solvent and temperature. Foregoing literature expounded the action of dichloroacetic acid (DCAA) as acid solvent in homogeneous reaction. However, DCAA is expensive and noxious, therefore, in the present paper cheap and non-noxious formic acid (88%) was in place of DCAA. Furthermore, during reaction formic acid was not dehydrated. Under formic acid we obtained the satisfying results that was higher yield and equivalent sulfur contents compared to DCAA. IR and (13)C NMR spectrums proved the structure of the resultant obtained under formic acid or DCAA to be same. Now, it has not been reported for formic acid as acid solvent in homogeneous reaction of chitosan sulfatation. In this present paper, we also determined antioxidant activity of high-molecular weight and high-sulfate-content chitosans (HCTS). The results showed that HCTS could scavenge superoxide and hydroxyl radical. Its IC(50) is 0.012 and 3.269 mg/mL, respectively. It had obviously reducing power and slight chelating activity. The data obtained in in vitro models clearly establish the antioxidant potency of HCTS. It is a potential antioxidant in vitro. (C) 2005 Elsevier Ltd. All rights reserved.; The influence of molecular weight and substitution degree of sulfated polysaccharides on their biological activity is considered in majority of works involving the anticoagulant or antiviral properties of these substances. Therefore, the present paper describes the effect of preparation conditions of sulfated chitosans on their molecular weight and sulfur content, such as different reaction time, acid solvent and temperature. Foregoing literature expounded the action of dichloroacetic acid (DCAA) as acid solvent in homogeneous reaction. However, DCAA is expensive and noxious, therefore, in the present paper cheap and non-noxious formic acid (88%) was in place of DCAA. Furthermore, during reaction formic acid was not dehydrated. Under formic acid we obtained the satisfying results that was higher yield and equivalent sulfur contents compared to DCAA. IR and C-13 NMR spectrums proved the structure of the resultant obtained under formic acid or DCAA to be same. Now, it has not been reported for formic acid as acid solvent in homogeneous reaction of chitosan sulfatation. In this present paper, we also determined antioxidant activity of high-molecular weight and high-sulfate-content chitosans (HCTS). The results showed that HCTS could scavenge superoxide and hydroxyl radical. Its IC50 is 0.012 and 3.269 mg/mL, respectively. It had obviously reducing power and slight chelating activity. The data obtained in in vitro models clearly establish the antioxidant potency of HCTS. It is a potential antioxidant in vitro. (C) 2005 Elsevier Ltd. All rights reserved.
关键词	Chitosans High-molecular Weight High-sulfate-content Dichloroacetic Acid Formic Acid Reducing Power Chelating Activity Radical Scavenging Effect
学科领域	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
DOI	10.1016/j.carbpol.2005.04.007
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000231168000004
引用统计	被引频次：114[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3542
专题	海洋生物技术研发中心海洋环境工程技术研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xing, RE,Liu, S,Yu, HH,et al. Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro[J]. CARBOHYDRATE POLYMERS,2005,61(2):148-154.
APA	Xing, RE,Liu, S,Yu, HH,Guo, ZY,Li, Z,&Li, PC.(2005).Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro.CARBOHYDRATE POLYMERS,61(2),148-154.
MLA	Xing, RE,et al."Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro".CARBOHYDRATE POLYMERS 61.2(2005):148-154.

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