Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye

doi:10.1016/j.bmcl.2005.09.010

IOCAS-IR > 海洋生物技术研发中心

	Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye
	Li, CP ; Yu, HH; Liu, S ; Xing, RE; Guo, ZY ; Li, PC
	2005-12-15
发表期刊	BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN	0960-894X
卷号	15 期号:24 页码:5370-5374
文章类型	Article
摘要	In this paper, the effects of some chemical and physical factors such as temperature, pH values, glycerol, and divalent metal cations on the protease activity of venom from jellyfish, Rhopilema esculentum Kishinouye, were assayed. Protease activity was dependent on temperature and pH values. Zn(2+), Mg(2+), and Mn(2+) in sodium phosphate buffer (0.02 M, pH 8.0) could increase protease activity. Mn(2+) had the best effects among the three metal cations and the effect was about 20 times of that of Zn(2+) or Mg(2+) and its maximal protease activity was 2.3 x 10(5) U/mL. EDTA could increase protease activity. PMSF had hardly affected protease activity. O-Phenanthroline and glycerol played an important part in inhibiting protease activity and their maximal inhibiting rates were 87.5% and 82.1%, respectively. (c) 2005 Elsevier Ltd. All rights reserved.; In this paper, the effects of some chemical and physical factors such as temperature, pH values, glycerol, and divalent metal cations on the protease activity of venom from jellyfish, Rhopilema esculentum Kishinouye, were assayed. Protease activity was dependent on temperature and pH values. Zn2+, Mg2+, and Mn2+ in sodium phosphate buffer (0.02 M, pH 8.0) could increase protease activity. Mn2+ had the best effects among the three metal cations and the effect was about 20 times of that of Zn2+ or Mg2+ and its maximal protease activity was 2.3 x 10(5) U/mL. EDTA could increase protease activity. PMSF had hardly affected protease activity. O-Phenanthroline and glycerol played an important part in inhibiting protease activity and their maximal inhibiting rates were 87.5% and 82.1%, respectively. (c) 2005 Elsevier Ltd. All rights reserved.
关键词	Jellyfish Protease Activity Rhopilema Esculentum Kishinouye Venom
学科领域	Chemistry, Medicinal ; Chemistry, Organic
DOI	10.1016/j.bmcl.2005.09.010
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000233206300002
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3514
专题	海洋生物技术研发中心海洋环境工程技术研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, CP,Yu, HH,Liu, S,et al. Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2005,15(24):5370-5374.
APA	Li, CP,Yu, HH,Liu, S,Xing, RE,Guo, ZY,&Li, PC.(2005).Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,15(24),5370-5374.
MLA	Li, CP,et al."Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 15.24(2005):5370-5374.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
li-Factors affecting（130KB）			限制开放	--	浏览