| Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye | |
Feng Jinhua1,2; Yu Huahua1 ; Li Cuiping1,2; Xing Ronge1; Liu Song1; Wang Lin1,2; Cai Shengbao1,2; Li Pengcheng1
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| 2009-11-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 卷号 | 27期号:4页码:869-874 |
| 文章类型 | Article |
| 摘要 | The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 A mu g/mL.; The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 A mu g/mL. |
| 关键词 | Nematocyst Jellyfish Venom Rhopilema Esculentum Hemolytic Activity |
| 学科领域 | Limnology ; Oceanography |
| DOI | 10.1007/s00343-009-9225-9 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272422300023 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/3470 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Feng Jinhua,Yu Huahua,Li Cuiping,et al. Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(4):869-874. |
| APA | Feng Jinhua.,Yu Huahua.,Li Cuiping.,Xing Ronge.,Liu Song.,...&Li Pengcheng.(2009).Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(4),869-874. |
| MLA | Feng Jinhua,et al."Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.4(2009):869-874. |
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