Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Biological effect of R-phycoerythrin-mediated photosensitization on DNA. | |
Li, GW; Li, ZG | |
2000 | |
发表期刊 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS |
ISSN | 1000-3282 |
卷号 | 27期号:6页码:621-624 |
摘要 | R-phycoerythrin (R-PE) is one of important proteins involved in capturing light during photosynthesis in red algae, and it is highly fluorescent, and water-soluble chromophores. In vivo, it can transfer the light energy into photosynthetic center, however, it can deliver the captured light energy captured to the surrounding oxygen in vitro and produce reactive oxygen species such as singlet oxygen, which is toxic to tumor cells. R-PE was added to the culture medium of tumor cells, subsequently with irradiation of 488 nm, Argon laser of 25.6 J/cm(2). The result by MTT assay showed that the survival rate decreased with the increase of R-PE concentration from 1 to 100 mg/L. The result from H-3-TdR incorporation demonstrated that the synthesis of DNA reduced when the concentration of R-PE increased from 0.01 to 0.32 mg/L. Besides, pUC18 DNA showed a conversion from supercoiled into linear conformation. The conclusion comes that R-PE mediated PDT can influence the conformation of DNA, and it may be one of the mechanisms of R-PE mediated photodynamic therapy. |
学科领域 | Biochemistry & Molecular Biology ; Biophysics |
收录类别 | sci |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/3342 |
专题 | 实验海洋生物学重点实验室 |
推荐引用方式 GB/T 7714 | Li, GW,Li, ZG. Biological effect of R-phycoerythrin-mediated photosensitization on DNA.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2000,27(6):621-624. |
APA | Li, GW,&Li, ZG.(2000).Biological effect of R-phycoerythrin-mediated photosensitization on DNA..PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,27(6),621-624. |
MLA | Li, GW,et al."Biological effect of R-phycoerythrin-mediated photosensitization on DNA.".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 27.6(2000):621-624. |
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