A centromeric satellite sequence in the Pacific oyster (Crassostrea gigas Thunberg) identified by fluorescence in situ hybridization

IOCAS-IR > 实验海洋生物学重点实验室

	A centromeric satellite sequence in the Pacific oyster (Crassostrea gigas Thunberg) identified by fluorescence in situ hybridization
	Wang, YP ; Xu, Z ; Guo, XM
	2001-09-01
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
卷号	3 期号:5 页码:486-492
文章类型	Article
摘要	A highly repetitive satellite sequence was previously identified in the Pacific oyster Crassostrea gigas Thunberg. The sequence has 168 bp per unit, present in tandem repeats, and accounts for 1% to 4% of the genome. We studied the chromosomal location of this satellite sequence by fluorescence in situ hybridization (FISH), A probe was made by polymerase chain reaction and incorporation of digoxigenin-11-dUTP. Hybridization was detected with fluorescein-labeled antidigoxigenin antibodies. FISH signals were located at centromeric regions of 7 pairs of the Pacific oyster chromosomes. No interstitial site was found. Signals were strong and consistent on chromosomes 1, 2, 4, and 7, but weak or variable oil chromosomes 5, 8, and 10. No signal was observed on chromosomes 3, 6, and 9. Our results showed that this sequence is clearly a centromeric satellite, disputing its previous assignment to the telomeric and submetacentric regions of 2 chromosomes. No signal was detected in the American oyster (Crassostrea virginica Gmelin).; A highly repetitive satellite sequence was previously identified in the Pacific oyster Crassostrea gigas Thunberg. The sequence has 168 bp per unit, present in tandem repeats, and accounts for 1% to 4% of the genome. We studied the chromosomal location of this satellite sequence by fluorescence in situ hybridization (FISH), A probe was made by polymerase chain reaction and incorporation of digoxigenin-11-dUTP. Hybridization was detected with fluorescein-labeled antidigoxigenin antibodies. FISH signals were located at centromeric regions of 7 pairs of the Pacific oyster chromosomes. No interstitial site was found. Signals were strong and consistent on chromosomes 1, 2, 4, and 7, but weak or variable oil chromosomes 5, 8, and 10. No signal was observed on chromosomes 3, 6, and 9. Our results showed that this sequence is clearly a centromeric satellite, disputing its previous assignment to the telomeric and submetacentric regions of 2 chromosomes. No signal was detected in the American oyster (Crassostrea virginica Gmelin).
关键词	Fish Chromosomes Repetitive Sequence Centromeric Satellite Crassostrea Gigas
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000172433600010
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3324
专题	实验海洋生物学重点实验室
作者单位	1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Post Norris, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, YP,Xu, Z,Guo, XM. A centromeric satellite sequence in the Pacific oyster (Crassostrea gigas Thunberg) identified by fluorescence in situ hybridization[J]. MARINE BIOTECHNOLOGY,2001,3(5):486-492.
APA	Wang, YP,Xu, Z,&Guo, XM.(2001).A centromeric satellite sequence in the Pacific oyster (Crassostrea gigas Thunberg) identified by fluorescence in situ hybridization.MARINE BIOTECHNOLOGY,3(5),486-492.
MLA	Wang, YP,et al."A centromeric satellite sequence in the Pacific oyster (Crassostrea gigas Thunberg) identified by fluorescence in situ hybridization".MARINE BIOTECHNOLOGY 3.5(2001):486-492.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
wang-A centromeric s（831KB）			限制开放	--	浏览