Expression of human epidermal growth factor gene in cyanobacteria

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	Expression of human epidermal growth factor gene in cyanobacteria
	Dai, W ; Shi, DJ ; Zhang, H; Zhong, H ; Ran, L ; Peng, GH ; Gan, RB ; Chen, SJ ; Lian, ML
	2001-12-01
发表期刊	ACTA BOTANICA SINICA
ISSN	0577-7496
卷号	43 期号:12 页码:1260-1264
文章类型	Article
摘要	The human epidermal growth factor (hEGF) is a small single-chain polypeptide of 53 amino acid residues. It can stimulate the proliferation of many cell types, mainly those of epidermal and epithelial tissues both in vivo and in vitro. A vector pRL-hEGF was constructed using plasmids pRL-489 and pUC-hEGF. The synthetic hEGF gene was recombined into the downstream of strong promoter psbA in plasmids pRL-489. Then, the vector was introduced into Synechococcus sp. PCC 7002 and Anabaena sp. PCC 7120 by triparental conjugative transfer. The transformation was confirmed by PCR amplification. The pRL-hEGF is thought to be retained as a plasmid form in the transgenic Anabaena sp. PCC 7120, since it can be recovered. However, it has been integrated into the chromosome of Synechococcus sp. PCC 7002 as there is no duplication origin in the pRL-hEGF in this cyanobacterium. and plasmid cannot be isolated from the Synechococcus sp. PCC 7002 either. The radioimmunoassay (RIA) proved that the hEGF gene has been expressed as the protein existed in these two strains of transgenic cyanobacteria, and the hEGF protein in Anabaena sp. PCC 7002 could be secreted into the medium.; The human epidermal growth factor (hEGF) is a small single-chain polypeptide of 53 amino acid residues. It can stimulate the proliferation of many cell types, mainly those of epidermal and epithelial tissues both in vivo and in vitro. A vector pRL-hEGF was constructed using plasmids pRL-489 and pUC-hEGF. The synthetic hEGF gene was recombined into the downstream of strong promoter psbA in plasmids pRL-489. Then, the vector was introduced into Synechococcus sp. PCC 7002 and Anabaena sp. PCC 7120 by triparental conjugative transfer. The transformation was confirmed by PCR amplification. The pRL-hEGF is thought to be retained as a plasmid form in the transgenic Anabaena sp. PCC 7120, since it can be recovered. However, it has been integrated into the chromosome of Synechococcus sp. PCC 7002 as there is no duplication origin in the pRL-hEGF in this cyanobacterium. and plasmid cannot be isolated from the Synechococcus sp. PCC 7002 either. The radioimmunoassay (RIA) proved that the hEGF gene has been expressed as the protein existed in these two strains of transgenic cyanobacteria, and the hEGF protein in Anabaena sp. PCC 7002 could be secreted into the medium.
关键词	Human Epidermal Growth Factor Anabaena Sp Pcc 7120 Synechococcus Sp Pcc 7002 Vector Transgenic Cyanobacteria Triparental Conjugative Transfer
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000173098900010
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3310
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Beijing Normal Univ, Dept Biol, Beijing 100875, Peoples R China 3.301 Hosp Peoples Liberat Army, Dongya Inst Immun, Beijing 100853, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Dai, W,Shi, DJ,Zhang, H,et al. Expression of human epidermal growth factor gene in cyanobacteria[J]. ACTA BOTANICA SINICA,2001,43(12):1260-1264.
APA	Dai, W.,Shi, DJ.,Zhang, H.,Zhong, H.,Ran, L.,...&Lian, ML.(2001).Expression of human epidermal growth factor gene in cyanobacteria.ACTA BOTANICA SINICA,43(12),1260-1264.
MLA	Dai, W,et al."Expression of human epidermal growth factor gene in cyanobacteria".ACTA BOTANICA SINICA 43.12(2001):1260-1264.

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