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Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome
Zhang, Hao1,2; Li, Fuchao2; Chen, Huaxin2; Zhao, Jin2; Yan, Jinfei3; Jiang, Peng2; Li, Ronggui1; Zhu, Baoli4
2015-07-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume33Issue:4Pages:819-827
SubtypeArticle
AbstractLipolytic enzymes, including esterases and lipases, represent a group of hydrolases that catalyze the cleavage and formation of ester bonds. A novel esterase gene, scsEst01, was cloned from a South China Sea sediment metagenome. The scsEst01 gene consisted of 921 bp encoding 307 amino acid residues. The predicted amino acid sequence shared less than 90% identity with other lipolytic enzymes in the NCBI nonredundant protein database. ScsEst01 was successfully co-expressed in Escherichia coli BL21 (DE3) with chaperones (dnaK-dnaJ-grpE) to prevent the formation of inclusion bodies. The recombinant protein was purified on an immobilized metal ion affinity column containing chelating Sepharose charged with Ni2+. The enzyme was characterized using p -nitrophenol butyrate as a substrate. ScsEst01 had the highest lipolytic activity at 35A degrees C and pH 8.0, indicative of a meso-thermophilic alkaline esterase. ScsEst01 was thermostable at 20A degrees C. The lipolytic activity of scsEst01 was strongly increased by Fe2+, Mn2+ and 1% Tween 80 or Tween 20.
KeywordMarine Sediment Metagenome Lipolytic Expression Esterase Characterization
DOI10.1007/s00343-015-4170-2
Indexed BySCI
Language英语
WOS IDWOS:000357037100001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/33091
Collection实验海洋生物学重点实验室
Affiliation1.Qingdao Univ, Microbiol Lab, Qingdao 266071, Shandong Provin, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.State Ocean Adm, Inst Oceanog 3, Xiamen 361005, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Hao,Li, Fuchao,Chen, Huaxin,et al. Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(4):819-827.
APA Zhang, Hao.,Li, Fuchao.,Chen, Huaxin.,Zhao, Jin.,Yan, Jinfei.,...&Zhu, Baoli.(2015).Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(4),819-827.
MLA Zhang, Hao,et al."Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.4(2015):819-827.
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