Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome

doi:10.1007/s00343-015-4170-2

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	Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome
	Zhang, Hao 1,2; Li, Fuchao 2; Chen, Huaxin 2; Zhao, Jin 2; Yan, Jinfei 3; Jiang, Peng 2; Li, Ronggui 1; Zhu, Baoli 4
	2015-07-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	33 期号:4 页码:819-827
文章类型	Article
摘要	Lipolytic enzymes, including esterases and lipases, represent a group of hydrolases that catalyze the cleavage and formation of ester bonds. A novel esterase gene, scsEst01, was cloned from a South China Sea sediment metagenome. The scsEst01 gene consisted of 921 bp encoding 307 amino acid residues. The predicted amino acid sequence shared less than 90% identity with other lipolytic enzymes in the NCBI nonredundant protein database. ScsEst01 was successfully co-expressed in Escherichia coli BL21 (DE3) with chaperones (dnaK-dnaJ-grpE) to prevent the formation of inclusion bodies. The recombinant protein was purified on an immobilized metal ion affinity column containing chelating Sepharose charged with Ni2+. The enzyme was characterized using p -nitrophenol butyrate as a substrate. ScsEst01 had the highest lipolytic activity at 35A degrees C and pH 8.0, indicative of a meso-thermophilic alkaline esterase. ScsEst01 was thermostable at 20A degrees C. The lipolytic activity of scsEst01 was strongly increased by Fe2+, Mn2+ and 1% Tween 80 or Tween 20.
关键词	Marine Sediment Metagenome Lipolytic Expression Esterase Characterization
DOI	10.1007/s00343-015-4170-2
收录类别	SCI
语种	英语
WOS记录号	WOS:000357037100001
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/33091
专题	实验海洋生物学重点实验室
作者单位	1.Qingdao Univ, Microbiol Lab, Qingdao 266071, Shandong Provin, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.State Ocean Adm, Inst Oceanog 3, Xiamen 361005, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Hao,Li, Fuchao,Chen, Huaxin,et al. Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(4):819-827.
APA	Zhang, Hao.,Li, Fuchao.,Chen, Huaxin.,Zhao, Jin.,Yan, Jinfei.,...&Zhu, Baoli.(2015).Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(4),819-827.
MLA	Zhang, Hao,et al."Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.4(2015):819-827.

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