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Functional Diversity of Anti-Lipopolysaccharide Factor Isoforms in Shrimp and Their Characters Related to Antiviral Activity
Li, Shihao1,2; Guo, Shuyue1,3; Li, Fuhua1,2; Xiang, Jianhai1
2015-05-01
发表期刊MARINE DRUGS
卷号13期号:5页码:2602-2616
文章类型Article
摘要Anti-lipopolysaccharide factor (ALF) is a small protein with broad-spectrum antimicrobial activity, which has potential application in the disease control. Previously, we isolated seven ALF isoforms from the Chinese shrimp Fenneropenaeuschinensis. In the present study, their distributions in tissues of shrimp were analyzed and the data showed that different isoforms had different expression profiles, which suggested that they might have different functions. Then, the functions of different isoforms were studied by analyzing the antibacterial and antiviral activities of the functional domain of ALFs, the LPS-binding domain (LBD), which were synthesized by chemical methods. Different ALFs showed distinct antibacterial and antiviral activities, which were consistent with their diverse tissue distribution patterns. Sequence analysis on the LBD domain of different isoforms revealed that an identical lysine residue site was specifically conserved in peptides with anti-WSSV activity. In order to confirm whether this lysine residue is critical to the antiviral activity of the peptide, new peptides were synthesized by changing residues at this site. Changing the lysine residue at the specific site to other amino acid residue, the antiviral activity of the peptide apparently decreased. While replacing other residue with a lysine residue at this site in LBD peptide without anti-WSSV activity, the peptide will obtain the antiviral activity to WSSV. These results not only showed us a comprehensive understanding on the function of ALFs from F. chinensis, but also provided clues for the development of ALFs as potential therapeutic drugs to WSSV.
关键词Anti-lipopolysaccharide Factor Lps-binding Domain Antibacterial Antiviral Lysine Residue
DOI10.3390/md13052602
收录类别SCI
语种英语
WOS记录号WOS:000356241100003
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/33080
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Li, Shihao,Guo, Shuyue,Li, Fuhua,et al. Functional Diversity of Anti-Lipopolysaccharide Factor Isoforms in Shrimp and Their Characters Related to Antiviral Activity[J]. MARINE DRUGS,2015,13(5):2602-2616.
APA Li, Shihao,Guo, Shuyue,Li, Fuhua,&Xiang, Jianhai.(2015).Functional Diversity of Anti-Lipopolysaccharide Factor Isoforms in Shrimp and Their Characters Related to Antiviral Activity.MARINE DRUGS,13(5),2602-2616.
MLA Li, Shihao,et al."Functional Diversity of Anti-Lipopolysaccharide Factor Isoforms in Shrimp and Their Characters Related to Antiviral Activity".MARINE DRUGS 13.5(2015):2602-2616.
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