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Edwardsiella tarda-regulated proteins in Japanese flounder (Paralichthys olivaceus): Identification and evaluation of antibacterial potentials
Wang, Jing-jing1,3; Sun, Li1,2
2015-06-21
发表期刊JOURNAL OF PROTEOMICS
卷号124页码:1-10
文章类型Article
摘要Edwardsiella tarda is a severe aquaculture pathogen that infects a wide range of fish including Japanese flounder (Paralichthys olivaceus). In this study, proteomic analysis was conducted to examine flounder spleen proteins altered in expression during E. tarda infection. Twenty differentially expressed proteins were identified, which belonged to five functional categories. Five upregulated proteins, i.e. calmodulin (Cam), cathepsin L (CatL), calreticulin (Crt), ferritin middle subunit (FerM), and natural killer enhancing factor (NKEF), were evaluated for antibacterial potential. For this purpose, Cam, CatL, Crt, FerM, and NKEF were each overexpressed in flounder, and the ensuing effect on E. tarda infection was assessed. The results showed that overexpression of these proteins, in particular CatL, Crt, FerM, and NKEF, significantly inhibited bacterial dissemination in and colonization of fish tissues. To further examine their effects on E. tarda infection, CatL, Crt, FerM, and NKEF were knocked down in vivo. Subsequent analysis showed that knockdown of each of these genes significantly enhanced E. tarda invasion in flounder. These results indicate that E. tarda infection induced expressional changes in the proteins of diverse functions in flounder, and that some of these proteins function as immune defense factors that are required for effective clearance of invading E. tarda.
关键词Edwardsiella Tarda Paralichthys Olivaceus Proteomic Antibacterial
DOI10.1016/j.jprot.2015.04.011
收录类别SCI
语种英语
WOS记录号WOS:000356735200001
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/33076
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Zhejiang Univ, Collaborat Innovat Ctr Deep Sea Biol, Hangzhou 310027, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Jing-jing,Sun, Li. Edwardsiella tarda-regulated proteins in Japanese flounder (Paralichthys olivaceus): Identification and evaluation of antibacterial potentials[J]. JOURNAL OF PROTEOMICS,2015,124:1-10.
APA Wang, Jing-jing,&Sun, Li.(2015).Edwardsiella tarda-regulated proteins in Japanese flounder (Paralichthys olivaceus): Identification and evaluation of antibacterial potentials.JOURNAL OF PROTEOMICS,124,1-10.
MLA Wang, Jing-jing,et al."Edwardsiella tarda-regulated proteins in Japanese flounder (Paralichthys olivaceus): Identification and evaluation of antibacterial potentials".JOURNAL OF PROTEOMICS 124(2015):1-10.
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