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CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin
Yang, Jialong1,3; Huang, Mengmeng1,3; Zhang, Huan1; Wang, Lingling1; Wang, Hao1; Wang, Leilei1,3; Qiu, Limei1; Song, Linsheng1,2
2015-05-15
Source PublicationSCIENTIFIC REPORTS
Volume5
SubtypeArticle
AbstractA C-type lectin (CfLec-3) from Chlamys farreri with three carbohydrate-recognition domains (CRDs) was selected to dissect the possible mechanisms of PAMP binding and functional differentiation of invertebrate lectins. CfLec-3 distributed broadly, and its mRNA expression in hemocytes increased significantly after stimulations with LPS, PGN or beta-glucan, but not poly(I: C). The recombinant CfLec-3 (rCfLec-3) could bind PAMPs and several microbes. rCfLec-3 mediated hemocytes phagocytosis against Escherichia coli and encapsulation towards agarose beads. Obvious functional differentiation occurred among the three CRDs, as CRD1 exhibited higher activity to bind PAMPs, while CRD2/3 were expert in promoting hemocyte mediated opsonisation. The tertiary structural differences were suspected to be associated with such functional differentiation. PAMP binding abilities of CfLec-3 were determined by Ca2+-binding site 2 motif. When Pro in this motif of each CRD was mutated into Ser, their PAMP binding abilities were deprived absolutely. rCRD2 acquired mannan binding capability when its EPD was replaced by EPN, but lost when EPN in rCRD3 was changed into EPD. The Pro in Ca2+-binding site 2 was indispensable for PAMPs binding, while Asn was determinant for specific binding to mannan. It shed new insight into PAMPs binding mechanism of invertebrate C-type lectins and their functional differentiation.
DOI10.1038/srep10068
Indexed BySCI
Language英语
WOS IDWOS:000355247500002
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/33045
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Dalian Ocean Univ, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Jialong,Huang, Mengmeng,Zhang, Huan,et al. CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin[J]. SCIENTIFIC REPORTS,2015,5.
APA Yang, Jialong.,Huang, Mengmeng.,Zhang, Huan.,Wang, Lingling.,Wang, Hao.,...&Song, Linsheng.(2015).CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin.SCIENTIFIC REPORTS,5.
MLA Yang, Jialong,et al."CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin".SCIENTIFIC REPORTS 5(2015).
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