beta-catenin in early development of the lancelet embryo indicates specific determination of embryonic polarity

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	beta-catenin in early development of the lancelet embryo indicates specific determination of embryonic polarity
	Yasui, K ; Li, GR ; Wang, Y; Saiga, H ; Zhang, PJ ; Aizawa, S
	2002-12-01
发表期刊	DEVELOPMENT GROWTH & DIFFERENTIATION
ISSN	0012-1592
卷号	44 期号:6 页码:467-475
文章类型	Article
摘要	The lancelet (amphioxus) embryo develops from a miolecithal egg and starts gastrulation when it is approximately 400 cells in size, in a fashion similar to that of some non-chordate deuterostomes. Throughout this type of gastrulation, the embryo develops characteristics such as the notochord and hollow nerve cord that commonly appear in chordates. beta-Catenin is an important factor in initiating body patterning. The behavior and developmental pattern of this protein in early lancelet development was examined in this study. Cytoplasmic beta-catenin was localized to the animal pole after fertilization and then was incorporated asymmetrically into the blastomeres during the first cleavage. Asymmetric distribution was observed at least until the 32-cell stage. The first nuclear localization was at the 64-cell stage, and involved all of the cells. At the initial gastrula stage, however, concentrated beta-catenin was found on the dorsal side. LiCl treatment affected the asymmetric pattern of beta-catenin during the first cleavage. LiCl also changed distribution of nuclear beta-catenin at the initial gastrula stage: distribution extended to cells on the animal side. Apparently associated with this change, expression domains of goosecoid, lhx3 and otx also changed to a radially symmetric pattern centered at the animal pole. However, LiCl-treated embryos were able to establish embryonic polarity. The present study suggests that in the lancelet embryo, polarity determination is independent of dorsal morphogenesis.; The lancelet (amphioxus) embryo develops from a miolecithal egg and starts gastrulation when it is approximately 400 cells in size, in a fashion similar to that of some non-chordate deuterostomes. Throughout this type of gastrulation, the embryo develops characteristics such as the notochord and hollow nerve cord that commonly appear in chordates. beta-Catenin is an important factor in initiating body patterning. The behavior and developmental pattern of this protein in early lancelet development was examined in this study. Cytoplasmic beta-catenin was localized to the animal pole after fertilization and then was incorporated asymmetrically into the blastomeres during the first cleavage. Asymmetric distribution was observed at least until the 32-cell stage. The first nuclear localization was at the 64-cell stage, and involved all of the cells. At the initial gastrula stage, however, concentrated beta-catenin was found on the dorsal side. LiCl treatment affected the asymmetric pattern of beta-catenin during the first cleavage. LiCl also changed distribution of nuclear beta-catenin at the initial gastrula stage: distribution extended to cells on the animal side. Apparently associated with this change, expression domains of goosecoid, lhx3 and otx also changed to a radially symmetric pattern centered at the animal pole. However, LiCl-treated embryos were able to establish embryonic polarity. The present study suggests that in the lancelet embryo, polarity determination is independent of dorsal morphogenesis.
关键词	Amphioxus Beta-catenin Chordate Dorsal Determination Embryonic Polarity Lithium Maternal Factor
学科领域	Cell Biology ; Developmental Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000179534700001
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3300
专题	实验海洋生物学重点实验室海洋环境腐蚀与生物污损重点实验室
作者单位	1.Kumamoto Univ, Inst Mol Embryol & Genet, Div Dev & Biohist, Kumamoto 8600811, Japan 2.Tokyo Metropolitan Univ, Grad Sch Sci, Dept Biol Sci, Tokyo 1920397, Japan 3.RIKEN Ctr Dev Biol, Vertebrate Body Plan Grp, Chuo Ku, Kobe, Hyogo 6500047, Japan 4.Shandong Teachers Univ, Dept Biol, Shadong 250014, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Shandong 266071, Peoples R China
推荐引用方式 GB/T 7714	Yasui, K,Li, GR,Wang, Y,et al. beta-catenin in early development of the lancelet embryo indicates specific determination of embryonic polarity[J]. DEVELOPMENT GROWTH & DIFFERENTIATION,2002,44(6):467-475.
APA	Yasui, K,Li, GR,Wang, Y,Saiga, H,Zhang, PJ,&Aizawa, S.(2002).beta-catenin in early development of the lancelet embryo indicates specific determination of embryonic polarity.DEVELOPMENT GROWTH & DIFFERENTIATION,44(6),467-475.
MLA	Yasui, K,et al."beta-catenin in early development of the lancelet embryo indicates specific determination of embryonic polarity".DEVELOPMENT GROWTH & DIFFERENTIATION 44.6(2002):467-475.

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