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Evidence for the involvement of cyclic AMP in the metamorphosis of bay scallop, Argopecten irradians (Lamarck) larvae
Zhang, T; Yang, HS; Que, HY; Zhang, GF; Liu, SL; He, YC; Zhang, FS
2003-09-01
发表期刊JOURNAL OF SHELLFISH RESEARCH
卷号22期号:2页码:403-408
文章类型Article
摘要The putative involvement of cyclic AMP (cAMP) in the metamorphosis of bay scallop Argopecten irradians larvae has been investigated on three integrated aspects. First, we conducted experiments on response of competent larvae to selective inhibitors of phosphodiesterase (PDE), theophylline, and caffeine, which presumably lead to elevated concentration of intracellular cAMP by preventing the degradation of cAMP to 5'-AMP. Second, the endogenous levels of cAMP were determined during larval development. Third, monitoring the variation of cAMP content in larvae when exposed to neuroactive compounds tested (L-DOPA and epinephrine) and to elevated concentrations of potassium ion, was carried out to examine the possible role of cAMP as a second messenger in metamorphic pathway stimulating artificially. Consistent results have been obtained in all the three experiments. The two putative PDE inhibitors that were tested stimulated metamorphosis in A. irradians larvae significantly above control level in a dosage-dependent manner. The inductive effects did not vary significantly with exposure time. At the optimum concentration of 1.0 mM, percent metamorphosis increased by 33% and 36.01% when subjected to theophylline and caffeine respectively. The endogenous level of cAMP varied dramatically over larval development. In particular, significant increase in cAMP content from 2129 pmol/(mg protein) for eyed larvae (Day 13 post-fertilization, PF) to 15,195 pmol/(mg protein) for spats (Day 17 PF) occurred during the metamorphic process. This finding indicates that metamorphic pathway involves cAMP in appreciable quantities. Furthermore, the endogenous cAMP content increased significantly in competent larvae exposed to excess potassium ion, epinephrine, or L-DOPA, suggesting cAMP plays an important role in metamorphic signal transduction pathway triggered by the three chemical cues. Evidences presented here show that cAMP becomes involved in the metamorphic pathway of A. irradians larvae.
关键词Camp Metamorphosis Argopecten Irradians Catecholamines L-dopa Pde Inhibitors
收录类别SCI
语种英语
WOS记录号WOS:000186096200006
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/33002
专题海洋生态与环境科学重点实验室
作者单位Chinese Acad Sci, Inst Oceanol, Shandong 266071, Peoples R China
推荐引用方式
GB/T 7714
Zhang, T,Yang, HS,Que, HY,et al. Evidence for the involvement of cyclic AMP in the metamorphosis of bay scallop, Argopecten irradians (Lamarck) larvae[J]. JOURNAL OF SHELLFISH RESEARCH,2003,22(2):403-408.
APA Zhang, T.,Yang, HS.,Que, HY.,Zhang, GF.,Liu, SL.,...&Zhang, FS.(2003).Evidence for the involvement of cyclic AMP in the metamorphosis of bay scallop, Argopecten irradians (Lamarck) larvae.JOURNAL OF SHELLFISH RESEARCH,22(2),403-408.
MLA Zhang, T,et al."Evidence for the involvement of cyclic AMP in the metamorphosis of bay scallop, Argopecten irradians (Lamarck) larvae".JOURNAL OF SHELLFISH RESEARCH 22.2(2003):403-408.
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