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Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita)
Que, H; Allen, SK
2002-06-01
Source PublicationJOURNAL OF SHELLFISH RESEARCH
Volume21Issue:1Pages:137-143
SubtypeArticle
AbstractThree replicates of hybrid crosses of tetraploid and diploid C gigas (Thunberg) with diploid C ariakensis (Fujita) were produced with controls. Larval survival and growth were documented. Cytological events were also monitored in oocytes from hybrid crosses following insemination. Among the four types of hybrid crosses, diploid C. gigas (female) x diploid C. ariakensis (male) (GA) was the most successful. Survival of GA was about the same as that of controls in two of three replications, although its growth rate was 25-30% lower. Crosses of tetraploid C. gigas (female) and diploid C. ariakensis (male) (GGA) had poor yield at day 2 post-fertilization (0.05%), but grew nearly as well as controls subsequently. The other two types of hybrids (i.e., diploid C. ariakensis [female] and tetraploid C. gigas [male] [AGG], diploid C ariakensis [female] and diploid C. gigos [male] [AG]) suffered very low yield at day 2 (0.01% and 0.003%) and grew very slowly. Spat were obtained from all replicates of GA crosses and one of three replicates of GGA, and proved to be hybrids by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) diagnosis. GGA hybrids were confirmed to be triploid by flow cytometry. No larvae survived to eyed stage in AGG or AG crosses. Cytological examination revealed that the vast majority (>99%) of oocytes from hybrid crosses had a prolonged meiotic prophase I or metaphase I at least through 180 min post-insemination.
KeywordCrassostrea Gigas Crassostrea Ariakensis Diploid Hybrid Tetraploid Oyster Breeding Polyploidy
Indexed BySCI
Language英语
WOS IDWOS:000176764900020
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32992
Collection海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
2.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
Recommended Citation
GB/T 7714
Que, H,Allen, SK. Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita)[J]. JOURNAL OF SHELLFISH RESEARCH,2002,21(1):137-143.
APA Que, H,&Allen, SK.(2002).Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita).JOURNAL OF SHELLFISH RESEARCH,21(1),137-143.
MLA Que, H,et al."Hybridization of tetraploid and diploid Crassostrea gigas (Thunberg) with diploid C-ariakensis (Fujita)".JOURNAL OF SHELLFISH RESEARCH 21.1(2002):137-143.
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