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Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization
Wang, YP; Guo, XM
2001-12-01
Source PublicationJOURNAL OF SHELLFISH RESEARCH
Volume20Issue:3Pages:1187-1190
SubtypeArticle
AbstractThe presence and chromosomal location of the vertebrate telomeric sequence (TTAGGG)n was studied in four bivalve molluscs: the eastern oyster, Crassostrea virginica Gmelin; the mangrove oyster, Crassostrea rhizophorae Guilding; the hard clam, Mercenaria mercenaria Linnaeus: and the dwarf surfclam, Mulinia lateralis Say, using fluorescence in situ hybridization (FISH). Metaphase chromosomes were prepared from embryos, and the vertebrate telomeric probe was labeled with digoxigenin and detected with fluorescein labeled anti-digoxigenin antibodies. Positive FISH signals were detected in all four species. Signals were restricted to telomeric regions of all chromosomes. and no interstitial sites were observed. Results of this and previous studies suggest that the vertebrate sequence is likely the telomeric sequence for all bivalve molluscs, which so far are the only invertebrates showing the vertebrate lineage in telomeric DNA.
KeywordFish Chromosome Telomeric Sequence Mapping Evolution Mollusca
Indexed BySCI
Language英语
WOS IDWOS:000174127300035
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32987
Collection实验海洋生物学重点实验室
Affiliation1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Wang, YP,Guo, XM. Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization[J]. JOURNAL OF SHELLFISH RESEARCH,2001,20(3):1187-1190.
APA Wang, YP,&Guo, XM.(2001).Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization.JOURNAL OF SHELLFISH RESEARCH,20(3),1187-1190.
MLA Wang, YP,et al."Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization".JOURNAL OF SHELLFISH RESEARCH 20.3(2001):1187-1190.
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