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Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization
Wang, YP; Guo, XM
2001-12-01
发表期刊JOURNAL OF SHELLFISH RESEARCH
卷号20期号:3页码:1187-1190
文章类型Article
摘要The presence and chromosomal location of the vertebrate telomeric sequence (TTAGGG)n was studied in four bivalve molluscs: the eastern oyster, Crassostrea virginica Gmelin; the mangrove oyster, Crassostrea rhizophorae Guilding; the hard clam, Mercenaria mercenaria Linnaeus: and the dwarf surfclam, Mulinia lateralis Say, using fluorescence in situ hybridization (FISH). Metaphase chromosomes were prepared from embryos, and the vertebrate telomeric probe was labeled with digoxigenin and detected with fluorescein labeled anti-digoxigenin antibodies. Positive FISH signals were detected in all four species. Signals were restricted to telomeric regions of all chromosomes. and no interstitial sites were observed. Results of this and previous studies suggest that the vertebrate sequence is likely the telomeric sequence for all bivalve molluscs, which so far are the only invertebrates showing the vertebrate lineage in telomeric DNA.
关键词Fish Chromosome Telomeric Sequence Mapping Evolution Mollusca
收录类别SCI
语种英语
WOS记录号WOS:000174127300035
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32987
专题实验海洋生物学重点实验室
作者单位1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Shandong, Peoples R China
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Wang, YP,Guo, XM. Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization[J]. JOURNAL OF SHELLFISH RESEARCH,2001,20(3):1187-1190.
APA Wang, YP,&Guo, XM.(2001).Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization.JOURNAL OF SHELLFISH RESEARCH,20(3),1187-1190.
MLA Wang, YP,et al."Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization".JOURNAL OF SHELLFISH RESEARCH 20.3(2001):1187-1190.
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