IOCAS-IR  > 实验海洋生物学重点实验室
Activation of Arp2/3 complex-mediated actin polymerization by cortactin
Uruno, T; Liu, JL; Zhang, PJ; Fan, YX; Egile, C; Li, P; Mueller, SC; Zhan, X
AbstractCortactin, a filamentous actin (F-actin)-associated protein and prominent substrate of Src, is implicated in progression of breast tumours through gene amplification at chromosome 11q13. However, the function of cortactin remains obscure. Here we show that cortactin co-localizes with the Arp2/3 complex, a de novo actin nucleator, at dynamic particulate structures enriched with actin filaments. Cortactin binds directly to the Arp2/3 complex and activates it to promote nucleation of actin filaments. The interaction of cortactin with the Arp2/3 complex occurs at an amino-terminal domain that is rich in acidic amino acids. Mutations in a conserved amino-acid sequence of DDW abolish both the interaction with the Arp2/3 complex and complex activation. The N-terminal domain is not only essential but also sufficient to target cortactin to actin-enriched patches within cells. Interestingly, the ability of cortactin to activate the Arp2/3 complex depends on an activity for F-actin binding; which is almost 20-fold higher than that of the Arp2/3 complex. Our data indicate a new mechanism for activation of actin polymerization involving an enhanced interaction between the Arp2/3 complex and actin filaments.
Indexed BySCI
WOS IDWOS:000167365800012
Citation statistics
Cited Times:466[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Amer Red Cross, Dept Expt Pathol, Holland Lab, Rockville, MD 20855 USA
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
4.Georgetown Univ, Sch Med, Dept Oncol, Washington, DC 20007 USA
5.George Washington Univ, Dept Cell Biol & Anat, Washington, DC 20037 USA
Recommended Citation
GB/T 7714
Uruno, T,Liu, JL,Zhang, PJ,et al. Activation of Arp2/3 complex-mediated actin polymerization by cortactin[J]. NATURE CELL BIOLOGY,2001,3(3):259-266.
APA Uruno, T.,Liu, JL.,Zhang, PJ.,Fan, YX.,Egile, C.,...&Zhan, X.(2001).Activation of Arp2/3 complex-mediated actin polymerization by cortactin.NATURE CELL BIOLOGY,3(3),259-266.
MLA Uruno, T,et al."Activation of Arp2/3 complex-mediated actin polymerization by cortactin".NATURE CELL BIOLOGY 3.3(2001):259-266.
Files in This Item:
File Name/Size DocType Version Access License
Activation of Arp2_3(797KB)期刊论文出版稿限制开放ODC PDDLView
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Uruno, T]'s Articles
[Liu, JL]'s Articles
[Zhang, PJ]'s Articles
Baidu academic
Similar articles in Baidu academic
[Uruno, T]'s Articles
[Liu, JL]'s Articles
[Zhang, PJ]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Uruno, T]'s Articles
[Liu, JL]'s Articles
[Zhang, PJ]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Activation of Arp2_3 complex-mediated actin polymerization by cortactin.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.