IOCAS-IR  > 实验海洋生物学重点实验室
A method for preserving oyster tissue samples for flow cytometry
Yang, HP; Gallivan, T; Guo, XM; Allen, SK
2000-12-01
发表期刊JOURNAL OF SHELLFISH RESEARCH
卷号19期号:2页码:835-839
文章类型Article
摘要Flow cytometry (FCM) is a powerful method for ploidy determination which has become important because of the increasing use of triploids in aquaculture. Tissue samples for FCM can be biopsied and kept fresh or frozen in a staining solution containing dimethyl sulfoxide (DMSO). Samples can be stored in the stain/DMSO at -80 degreesC indefinitely, or shipped on dry ice to a flow cytometry lab. But ultracold freezers and overnight shipping are not always available, for example, at rural labs and hatcheries. We investigated several methods of preserving FCM samples that do not involve freezing. Three different tissues, gill, mantle, and hemolymph from diploid and triploid pacific oysters, Crassostrea gigas Thunberg, were preserved by different methods, including pre-treatments and different fixatives. Gill was the best tissue for FCM analysis, and ethanol (75%) was the preffered fixative. Hypotonic treatments before fixation promoted nucleus-dissociation needed for FCM. The recommended protocol for preserving gill tissue is to dissect or biopsy a piece Bill tissue (similar to0.5 cm(2)), treat with 0.075 M KCl for 10 min and fix in 75% ethanol that is changed once. Before FCM, the fixed tissue is washed once using a phosphate buffered saline (pH = 6.8) and transferred to a staining buffer containing 10% DMSO. The stained sample is frozen and thawed, votexed. aspiarted, and filtered before analysis. This method can also be used for preserving D-stage larvae and gill tissue samples of other bivalve species.
关键词Triploidy Polyploidy Dna Content Flow Cytometry Fixation Oyster Mollusc
收录类别SCI
语种英语
WOS记录号WOS:000166380100019
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32970
专题实验海洋生物学重点实验室
作者单位1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Shandong 266071, Peoples R China
推荐引用方式
GB/T 7714
Yang, HP,Gallivan, T,Guo, XM,et al. A method for preserving oyster tissue samples for flow cytometry[J]. JOURNAL OF SHELLFISH RESEARCH,2000,19(2):835-839.
APA Yang, HP,Gallivan, T,Guo, XM,&Allen, SK.(2000).A method for preserving oyster tissue samples for flow cytometry.JOURNAL OF SHELLFISH RESEARCH,19(2),835-839.
MLA Yang, HP,et al."A method for preserving oyster tissue samples for flow cytometry".JOURNAL OF SHELLFISH RESEARCH 19.2(2000):835-839.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A method for preserv(1251KB)期刊论文出版稿开放获取ODC PDDL浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, HP]的文章
[Gallivan, T]的文章
[Guo, XM]的文章
百度学术
百度学术中相似的文章
[Yang, HP]的文章
[Gallivan, T]的文章
[Guo, XM]的文章
必应学术
必应学术中相似的文章
[Yang, HP]的文章
[Gallivan, T]的文章
[Guo, XM]的文章
相关权益政策
暂无数据
收藏/分享
文件名: A method for preserving oyster tissue samples for flow cytometry.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。