White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

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	White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis
	Wang, YT ; Liu, W ; Seah, JN ; Lam, CS ; Xiang, JH; Korzh, V ; Kwang, J
	2002-12-10
发表期刊	DISEASES OF AQUATIC ORGANISMS
ISSN	0177-5103
卷号	52 期号:3 页码:249-259
文章类型	Article
摘要	White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes-GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.; White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes-GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.
关键词	White Spot Syndrome Virus Wssv Penaeus Merguiensis Hemocyte Flow Cytometry Immunofluorescence Assay Ifa
学科领域	Fisheries ; Veterinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000180975400008
引用统计	被引频次：86[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3296
专题	实验海洋生物学重点实验室
作者单位	1.Natl Univ Singapore, Inst Mol Agrobiol, Singapore 117604, Singapore 2.Chinese Acad Sci, Inst Oceanol, Shandong 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, YT,Liu, W,Seah, JN,et al. White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis[J]. DISEASES OF AQUATIC ORGANISMS,2002,52(3):249-259.
APA	Wang, YT.,Liu, W.,Seah, JN.,Lam, CS.,Xiang, JH.,...&Kwang, J.(2002).White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis.DISEASES OF AQUATIC ORGANISMS,52(3),249-259.
MLA	Wang, YT,et al."White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis".DISEASES OF AQUATIC ORGANISMS 52.3(2002):249-259.

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