Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers

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	Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers
	Li, Sanlei 1; Xu, Dongdong 1; Lou, Bao 1; Xu, Hanxiang 1; You, Feng 2; Zhan, Wei 1; Geng, Zhi 1
	2011-10-05
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
卷号	10 期号:60 页码:12833-12839
文章类型	Article
摘要	The population structure of Japanese flounder (Paralichthys olivaceus) in the Yellow and East China Seas were analyzed using amplified fragment length polymorphism (AFLP) and cytochrome c oxidase subunit I (COI) gene sequencing. A total of 390 reproducible bands were generated by 10 AFLP primer combinations in two populations collected from the coasts of Qingdao (located at the Yellow Sea) and Zhoushan (located at the East China Sea). The percentage of polymorphic loci (P), Nei's genetic diversity (H) and Shannon's information index (I) values were higher in the Qingdao population (P = 72.85%, H = 0.243 and I = 0.364) than those in the Zhoushan population (P = 56.35%, H = 0.189 and I = 0.284). The genetic diversity reduction in the Zhoushan population may be attributed to fishing pressure and habitat loss in this area. Based on the COI sequencing analysis, a total of 25 polymorphic sites were examined, and 15 haplotypes were identified in the two populations. The haplotype diversity (h) and nucleotide diversity (pi) values in the Qingdao population were 0.746 +/- 0.0728 and 0.00334 +/- 0.00103, respectively. The corresponding values in the Zhoushan population were 0.712 +/- 0.0470 and 0.00318 +/- 0.00049. Both the AFLP and mtDNA data revealed significant genetic differentiation between the two populations. The present study discussed the factors that may result in genetic differentiation between the populations in the Yellow and East China Seas.
关键词	Japanese Flounder Amplified Fragment Length Polymorphism (Aflp) Cytochrome c Oxidase Subunit i (Coi) Gene Genetic Diversity Population Structure
收录类别	SCI
语种	英语
WOS记录号	WOS:000298544000006
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32944
专题	实验海洋生物学重点实验室
作者单位	1.Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China 2.Zhejiang Ocean Univ, Coll Marine Sci, Zhoushan 316100, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Sanlei,Xu, Dongdong,Lou, Bao,et al. Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(60):12833-12839.
APA	Li, Sanlei.,Xu, Dongdong.,Lou, Bao.,Xu, Hanxiang.,You, Feng.,...&Geng, Zhi.(2011).Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(60),12833-12839.
MLA	Li, Sanlei,et al."Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.60(2011):12833-12839.

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