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Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas
Wang, Xiaotong; Li, Li; Xu, Fei; Zhang, Guofan
2011
Source PublicationISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
Volume63
SubtypeArticle
AbstractAs opposed to other animals, live bivalve shellfish cannot be sampled because of the high mortality associated with opening closed shells. In this paper, we present a new anesthesia method using an MgSO4 seawater solution for sampling live bivalve shellfish. In our experiment, genomic DNA was successfully extracted from mantle tissues of sampled Pacific oyster (Crassostrea gigas) and the DNA was used as a template for polymerase chain reaction (PCR), which indicated that 3 mm(3) of mantle tissue was enough for DNA extraction and subsequent molecular biology testing. The survival rate after the in vivo sampling was high (83.3%), indicating that the sampling method caused only slight harm to the oysters. Thus, MgSO4 anesthesia is a practical method for in vivo sampling of oysters and probably could be applied to other bivalve shellfish species as well, significantly promoting the application of molecular marker-assisted selection in bivalve shellfish breeding and the study of tissue injury and repair mechanisms in bivalve shellfish.
KeywordOyster Live Sampling Genomic Dna Mgso4 Anesthesia
Indexed BySCI
Language英语
WOS IDWOS:000298952800001
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32932
Collection海洋生物技术研发中心
AffiliationChinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiaotong,Li, Li,Xu, Fei,et al. Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas[J]. ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,2011,63.
APA Wang, Xiaotong,Li, Li,Xu, Fei,&Zhang, Guofan.(2011).Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas.ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,63.
MLA Wang, Xiaotong,et al."Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas".ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH 63(2011).
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