IOCAS-IR  > 海洋生物技术研发中心
Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas
Wang, Xiaotong; Li, Li; Xu, Fei; Zhang, Guofan
2011
发表期刊ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
卷号63
文章类型Article
摘要As opposed to other animals, live bivalve shellfish cannot be sampled because of the high mortality associated with opening closed shells. In this paper, we present a new anesthesia method using an MgSO4 seawater solution for sampling live bivalve shellfish. In our experiment, genomic DNA was successfully extracted from mantle tissues of sampled Pacific oyster (Crassostrea gigas) and the DNA was used as a template for polymerase chain reaction (PCR), which indicated that 3 mm(3) of mantle tissue was enough for DNA extraction and subsequent molecular biology testing. The survival rate after the in vivo sampling was high (83.3%), indicating that the sampling method caused only slight harm to the oysters. Thus, MgSO4 anesthesia is a practical method for in vivo sampling of oysters and probably could be applied to other bivalve shellfish species as well, significantly promoting the application of molecular marker-assisted selection in bivalve shellfish breeding and the study of tissue injury and repair mechanisms in bivalve shellfish.
关键词Oyster Live Sampling Genomic Dna Mgso4 Anesthesia
收录类别SCI
语种英语
WOS记录号WOS:000298952800001
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32932
专题海洋生物技术研发中心
作者单位Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xiaotong,Li, Li,Xu, Fei,et al. Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas[J]. ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,2011,63.
APA Wang, Xiaotong,Li, Li,Xu, Fei,&Zhang, Guofan.(2011).Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas.ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,63.
MLA Wang, Xiaotong,et al."Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas".ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH 63(2011).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Genomic DNA Extracti(109KB)期刊论文出版稿开放获取ODC PDDL浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Xiaotong]的文章
[Li, Li]的文章
[Xu, Fei]的文章
百度学术
百度学术中相似的文章
[Wang, Xiaotong]的文章
[Li, Li]的文章
[Xu, Fei]的文章
必应学术
必应学术中相似的文章
[Wang, Xiaotong]的文章
[Li, Li]的文章
[Xu, Fei]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。