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Identification and immunoprotective analysis of a Streptococcus iniae subunit vaccine candidate
Cheng, Shuang1,2; Hu, Yong-hua1; Jiao, Xu-dong1,2; Sun, Li1
2010-03-19
Source PublicationVACCINE
Volume28Issue:14Pages:2636-2641
SubtypeArticle
AbstractStreptococcus iniae is a Gram-positive bacterium and a severe aquaculture pathogen that can infect a wide range of farmed fish species. In the summer of 2006, an epidemic broke out in a fish farm in north China, and examination of moribund fish (Japanese flounder) identified the possible etiological agent of the outbreak as a strain named SF1, which exhibited apparent virulence in a Japanese flounder infection model and conforms to the description of S. iniae by 16S rRNA sequence analysis and API 20 Strep test. Biochemical and random amplified polymorphic DNA analyses indicated that SF1 is of the serotype I. A putative iron-binding protein, Sip11, was identified from SF1 using a previously established molecular trap that selects exported proteins. Recombinant Sip11 was purified from Escherichia coli and found to be protective against SF1 infection when used as an injection vaccine administered intraperitoneally into Japanese flounder. To improve the vaccine potential of Sip11, an E. coli strain was constructed, which expresses and secrets recombinant Sip11 covalently linked to a carrier protein in the form of a chimera. Vaccination of Japanese flounder with live Sip11-secreting E. coli afforded complete protection upon the fish following lethal SF1 challenge. These results indicate that Sip11, especially when delivered by a live bacterial carrier, is an effective vaccine candidate against SF1 infection. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordBacterial Delivery Immunogen Streptococcus Iniae Vaccine
DOI10.1016/j.vaccine.2010.01.016
Indexed BySCI
Language英语
WOS IDWOS:000276505700008
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Cited Times:49[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32914
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Cheng, Shuang,Hu, Yong-hua,Jiao, Xu-dong,et al. Identification and immunoprotective analysis of a Streptococcus iniae subunit vaccine candidate[J]. VACCINE,2010,28(14):2636-2641.
APA Cheng, Shuang,Hu, Yong-hua,Jiao, Xu-dong,&Sun, Li.(2010).Identification and immunoprotective analysis of a Streptococcus iniae subunit vaccine candidate.VACCINE,28(14),2636-2641.
MLA Cheng, Shuang,et al."Identification and immunoprotective analysis of a Streptococcus iniae subunit vaccine candidate".VACCINE 28.14(2010):2636-2641.
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