IOCAS-IR  > 海洋生物分类与系统演化实验室
A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments
Xu, Kuidong1; Du, Yongfen1,2; Lei, Yanli1; Dai, Renhai1,2
2010-11-01
Source PublicationEUROPEAN JOURNAL OF PROTISTOLOGY
Volume46Issue:4Pages:263-270
SubtypeArticle
AbstractMethodological impediments have long been the main problem in estimating the ecological role of marine benthic ciliates. Percoll density centrifugation is currently the most efficient technique for extracting ciliates from fine-grained sediments, while the high cost and low density of Percoll limit its wide application. We developed a protocol of density gradient centrifugation using the cheap sol Ludox HS 40 in combination with the quantitative protargol stain (QPS) to enumerate and identify marine benthic ciliates. The combined Ludox-QPS method involves sample collection and salt reduction, extraction with Ludox centrifugation, and preparation with the QPS technique. The recovery efficiency of Ludox was first tested with azoic sandy and muddy sediments. A 94-100% recovery rate of ciliates was reached. The method was further tested with natural sandy, muddy-sand and muddy sediments. Excellent extraction efficiencies were consistently obtained: an average of 97.6% for ciliates in sand, and 96.9-97.8% for nematodes in the three types of sediments. The high efficiencies indicate that the method allows for simultaneous enumeration of micro- and meiobenthos. Advantages of the new method include: (i) reliable and cost-efficient operation; (ii) appropriate centrifugation for both micro- and meiobenthos; and (iii) applicability to large samples and routine ecological surveys. (C) 2010 Elsevier GmbH. All rights reserved.
KeywordProtozoa Ludox-qps Method Extraction Efficiency Identification Benthic Ciliates
DOI10.1016/j.ejop.2010.04.005
Indexed BySCI
Language英语
WOS IDWOS:000286498900003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32895
Collection海洋生物分类与系统演化实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xu, Kuidong,Du, Yongfen,Lei, Yanli,et al. A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2010,46(4):263-270.
APA Xu, Kuidong,Du, Yongfen,Lei, Yanli,&Dai, Renhai.(2010).A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments.EUROPEAN JOURNAL OF PROTISTOLOGY,46(4),263-270.
MLA Xu, Kuidong,et al."A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments".EUROPEAN JOURNAL OF PROTISTOLOGY 46.4(2010):263-270.
Files in This Item:
File Name/Size DocType Version Access License
A practical method o(422KB)期刊论文出版稿开放获取ODC PDDLView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Kuidong]'s Articles
[Du, Yongfen]'s Articles
[Lei, Yanli]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Kuidong]'s Articles
[Du, Yongfen]'s Articles
[Lei, Yanli]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Kuidong]'s Articles
[Du, Yongfen]'s Articles
[Lei, Yanli]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.