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A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments
Xu, Kuidong1; Du, Yongfen1,2; Lei, Yanli1; Dai, Renhai1,2
2010-11-01
发表期刊EUROPEAN JOURNAL OF PROTISTOLOGY
卷号46期号:4页码:263-270
文章类型Article
摘要Methodological impediments have long been the main problem in estimating the ecological role of marine benthic ciliates. Percoll density centrifugation is currently the most efficient technique for extracting ciliates from fine-grained sediments, while the high cost and low density of Percoll limit its wide application. We developed a protocol of density gradient centrifugation using the cheap sol Ludox HS 40 in combination with the quantitative protargol stain (QPS) to enumerate and identify marine benthic ciliates. The combined Ludox-QPS method involves sample collection and salt reduction, extraction with Ludox centrifugation, and preparation with the QPS technique. The recovery efficiency of Ludox was first tested with azoic sandy and muddy sediments. A 94-100% recovery rate of ciliates was reached. The method was further tested with natural sandy, muddy-sand and muddy sediments. Excellent extraction efficiencies were consistently obtained: an average of 97.6% for ciliates in sand, and 96.9-97.8% for nematodes in the three types of sediments. The high efficiencies indicate that the method allows for simultaneous enumeration of micro- and meiobenthos. Advantages of the new method include: (i) reliable and cost-efficient operation; (ii) appropriate centrifugation for both micro- and meiobenthos; and (iii) applicability to large samples and routine ecological surveys. (C) 2010 Elsevier GmbH. All rights reserved.
关键词Protozoa Ludox-qps Method Extraction Efficiency Identification Benthic Ciliates
DOI10.1016/j.ejop.2010.04.005
收录类别SCI
语种英语
WOS记录号WOS:000286498900003
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32895
专题海洋生物分类与系统演化实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
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Xu, Kuidong,Du, Yongfen,Lei, Yanli,et al. A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2010,46(4):263-270.
APA Xu, Kuidong,Du, Yongfen,Lei, Yanli,&Dai, Renhai.(2010).A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments.EUROPEAN JOURNAL OF PROTISTOLOGY,46(4),263-270.
MLA Xu, Kuidong,et al."A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments".EUROPEAN JOURNAL OF PROTISTOLOGY 46.4(2010):263-270.
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