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Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen
Jiao, Xu-dong1,2; Zhang, Min1; Cheng, Shuang1,2; Sun, Li1
2010-04-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号28期号:4页码:672-677
文章类型Article
摘要Edwardsiella tarda is a severe aquaculture pathogen with a broad host range that includes humans, animal, and fish. A gene (degP(Et)) encoding a DegP homologue was cloned from TX01, a pathogenic E. tarda strain isolated from diseased fish. DegP(Et) shares high sequence identities with the DegP proteins of several bacterial species. Functional analyses showed that degP(Et) could complement the temperature-sensitive phenotype of an Escherichia coli degP null mutant. Expression of degP(Et) in TX01 was modulated by growth phase and temperature, the latter possibly through the action of the sigma(E)-like factor. Overexpression of degP(Et) (i) enhanced the ability of TX01 to disseminate in fish blood at the advanced stage of infection. (ii) heightened the activity of type 2 autoinducer, and (iii) increased the expression of luxS and the genes encoding components of the virulence-associated type III secretion system. Recombinant DegP(Et) purified from E. coli was a serine protease that exhibited maximum activity at 40 degrees C and pH8.0. The proteolytic activity of recombinant DegP(Et) depended on the catalytic triad and the PDZ domains. Immunoprotective analyses showed that purified recombinant DegP(Et) was a protective immunogen that could induce the production of specific serum antibodies and elicit strong protective immunity in fish vaccinated with DegP(Et). (C) 2010 Elsevier Ltd. All rights reserved.
关键词Degp Edwardsiella Tarda Immunogen Luxs Protease Virulence
DOI10.1016/j.fsi.2010.01.004
收录类别SCI
语种英语
WOS记录号WOS:000276935700021
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32894
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Jiao, Xu-dong,Zhang, Min,Cheng, Shuang,et al. Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(4):672-677.
APA Jiao, Xu-dong,Zhang, Min,Cheng, Shuang,&Sun, Li.(2010).Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen.FISH & SHELLFISH IMMUNOLOGY,28(4),672-677.
MLA Jiao, Xu-dong,et al."Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen".FISH & SHELLFISH IMMUNOLOGY 28.4(2010):672-677.
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