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Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues
Zhang Yuqing1,2; Tan Xungang1; Xu Peng1,2; Sun Wei1,2; Xu Yongli1; Zhang Peijun1
2010-03-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume28Issue:2Pages:248-253
SubtypeArticle
AbstractMyoD, Myf5, and myogenin are myogenic regulatory factors that play important roles during myogenesis. It is thought that MyoD and Myf5 are required for myogenic determination, while myogenin is important for terminal differentiation and lineage maintenance. To better understand the function of myogenic regulatory factors in muscle development of flounder, an important economic fish in Asia, real-time quantitative RT-PCR was used to characterize the expression patterns of MyoD, Myf5, and myogenin at early stages of embryo development, and in different tissues of the adult flounder. The results show that, Myf5 is the first gene to be expressed during the early stages of flounder development, followed by MyoD and myogenin. The expressions of Myf5, yoD, and myogenin at the early stages have a common characteristic: expression gradually increased to a peak level, and then gradually decreased to an extremely low level. In the adult flounder, the expression of the three genes in muscle is much higher than that in other tissues, indicating that they are important for muscle growth and maintenance of grown fish. During embryonic stages, the expression level of MyoD might serve an important role in the balance between muscle cell differentiation and proliferation. When the MyoD expression is over 30% of its highest level, the muscle cells enter the differentiation stage.
KeywordParalichthys Olivaceus Myogenic Regulatory Factors Myogenesis Muscle Quantitative Rt-pcr
DOI10.1007/s00343-010-9286-9
Indexed BySCI
Language英语
WOS IDWOS:000275781400007
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32885
Collection实验海洋生物学重点实验室
海洋环流与波动重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang Yuqing,Tan Xungang,Xu Peng,et al. Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(2):248-253.
APA Zhang Yuqing,Tan Xungang,Xu Peng,Sun Wei,Xu Yongli,&Zhang Peijun.(2010).Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(2),248-253.
MLA Zhang Yuqing,et al."Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.2(2010):248-253.
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