IOCAS-IR  > 实验海洋生物学重点实验室
Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain
Cheng, Shuang1,2; Zhang, Wei-wei1,3; Zhang, Min1; Sun, Li1
2010-01-22
发表期刊VACCINE
卷号28期号:4页码:1041-1047
文章类型Article
摘要Vibrio harveyi is an important aquaculture pathogen that can infect a number of fish species and marine invertebrates. A putative protease. Vhp1, was identified front a pathogenic V harveyi strain isolated from diseased fish as a protein with secretion capacity Vhp1 is 530 amino acids in length and shares high sequence identities with several extracellular serine proteases of the Vibrio species In silico analysis identified a protease domain in Vhp1. which is preceded by a subtilisin-N domain and followed by a bacterial pre-peptidase C-terminal domain Purified recombinant protein corresponding to the protease domain of Vhp1 exhibited apparent proteolytic activity that was relatively heat-stable and reached maximum at pH 8 0 and 50 degrees C The activity of purified recombinant Vhp1 protease was enhanced by Ca2+ and inhibited by Mn2+ and ethylenedinitrilotetraacetic acid Cytotoxicity analyses indicated that recombinant Vhp1 protease was toxic to cultured Japanese flounder cells and could cause complete cell lysis. Immunoprotective analysis using Japanese flounder as an animal model showed that purified recombinant Vhp1 in the form of a denatured and proteolytically inactive protein was an effective subunit vaccine To improve the vaccine potential of Vhp1. an Escherichia coli strain that expresses and secrets a cytotoxically impaired Vhp1 was constructed. which, when used as a live vaccine, afforded a high level of protection upon the vaccinated fish against lethal V. harveyi challenge Taken together, these results demonstrate that Vhp1 is a cytotoxic protease and in effective vaccine candidate against V harveyi infection. (C) 2009 Elsevier Ltd All rights reserved
关键词Cytotoxin Immunoprotective Protease Vaccine Vibrio Harveyi
DOI10.1016/j.vaccine.2009.10.122
收录类别SCI
语种英语
WOS记录号WOS:000274943300029
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32874
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714
Cheng, Shuang,Zhang, Wei-wei,Zhang, Min,et al. Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain[J]. VACCINE,2010,28(4):1041-1047.
APA Cheng, Shuang,Zhang, Wei-wei,Zhang, Min,&Sun, Li.(2010).Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain.VACCINE,28(4),1041-1047.
MLA Cheng, Shuang,et al."Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain".VACCINE 28.4(2010):1041-1047.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Evaluation of the va(366KB)期刊论文出版稿开放获取ODC PDDL浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cheng, Shuang]的文章
[Zhang, Wei-wei]的文章
[Zhang, Min]的文章
百度学术
百度学术中相似的文章
[Cheng, Shuang]的文章
[Zhang, Wei-wei]的文章
[Zhang, Min]的文章
必应学术
必应学术中相似的文章
[Cheng, Shuang]的文章
[Zhang, Wei-wei]的文章
[Zhang, Min]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。