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Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain
Cheng, Shuang1,2; Zhang, Wei-wei1,3; Zhang, Min1; Sun, Li1
2010-01-22
Source PublicationVACCINE
Volume28Issue:4Pages:1041-1047
SubtypeArticle
AbstractVibrio harveyi is an important aquaculture pathogen that can infect a number of fish species and marine invertebrates. A putative protease. Vhp1, was identified front a pathogenic V harveyi strain isolated from diseased fish as a protein with secretion capacity Vhp1 is 530 amino acids in length and shares high sequence identities with several extracellular serine proteases of the Vibrio species In silico analysis identified a protease domain in Vhp1. which is preceded by a subtilisin-N domain and followed by a bacterial pre-peptidase C-terminal domain Purified recombinant protein corresponding to the protease domain of Vhp1 exhibited apparent proteolytic activity that was relatively heat-stable and reached maximum at pH 8 0 and 50 degrees C The activity of purified recombinant Vhp1 protease was enhanced by Ca2+ and inhibited by Mn2+ and ethylenedinitrilotetraacetic acid Cytotoxicity analyses indicated that recombinant Vhp1 protease was toxic to cultured Japanese flounder cells and could cause complete cell lysis. Immunoprotective analysis using Japanese flounder as an animal model showed that purified recombinant Vhp1 in the form of a denatured and proteolytically inactive protein was an effective subunit vaccine To improve the vaccine potential of Vhp1. an Escherichia coli strain that expresses and secrets a cytotoxically impaired Vhp1 was constructed. which, when used as a live vaccine, afforded a high level of protection upon the vaccinated fish against lethal V. harveyi challenge Taken together, these results demonstrate that Vhp1 is a cytotoxic protease and in effective vaccine candidate against V harveyi infection. (C) 2009 Elsevier Ltd All rights reserved
KeywordCytotoxin Immunoprotective Protease Vaccine Vibrio Harveyi
DOI10.1016/j.vaccine.2009.10.122
Indexed BySCI
Language英语
WOS IDWOS:000274943300029
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Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32874
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Cheng, Shuang,Zhang, Wei-wei,Zhang, Min,et al. Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain[J]. VACCINE,2010,28(4):1041-1047.
APA Cheng, Shuang,Zhang, Wei-wei,Zhang, Min,&Sun, Li.(2010).Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain.VACCINE,28(4),1041-1047.
MLA Cheng, Shuang,et al."Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain".VACCINE 28.4(2010):1041-1047.
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