Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis

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	Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis
	Sun, X; Yang, GP ; Mao, YX ; Zhang, XC ; Sui, ZH ; Qin, S
	2002-07-01
发表期刊	PROGRESS IN NATURAL SCIENCE
ISSN	1002-0071
卷号	12 期号:7 页码:518-523
文章类型	Article
摘要	The expressed sequence tags (EST) has been proved to be a useful tool for discovering and identifying functional genes, especially in some species whose genetic information is unavailable. A total of 180 ESTs have been generated from a cDNA library of gametophytic Gracilaria lemaneiformis in this study. These clones are clustered into 151 groups, among which 8 groups are highly homologous to chloroplast genes and are abundant in the library. After searching for matches in the EST database of red alga, 22 groups are found to match with the registered ESTs of Rhadophyta and 6 with Gracilaria. Searching in the protein database reveal that 73 non-redundant clones have significant similarity to some known sequences, the majority of which are involved in photosynthesis, DNA transcription or translation, and 6, 4 and 3 clones are associated with growth or development, signal transduction and stress or defense response, respectively.; The expressed sequence tags (EST) has been proved to be a useful tool for discovering and identifying functional genes, especially in some species whose genetic information is unavailable. A total of 180 ESTs have been generated from a cDNA library of gametophytic Gracilaria lemaneiformis in this study. These clones are clustered into 151 groups, among which 8 groups are highly homologous to chloroplast genes and are abundant in the library. After searching for matches in the EST database of red alga, 22 groups are found to match with the registered ESTs of Rhadophyta and 6 with Gracilaria. Searching in the protein database reveal that 73 non-redundant clones have significant similarity to some known sequences, the majority of which are involved in photosynthesis, DNA transcription or translation, and 6, 4 and 3 clones are associated with growth or development, signal transduction and stress or defense response, respectively.
关键词	Gracilaria Lemaneiformis Expressed Sequence Tags (Est) Homologous Sequence
学科领域	Multidisciplinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000176691600006
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3286
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
作者单位	1.Ocean Univ Qingdao, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, X,Yang, GP,Mao, YX,et al. Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis[J]. PROGRESS IN NATURAL SCIENCE,2002,12(7):518-523.
APA	Sun, X,Yang, GP,Mao, YX,Zhang, XC,Sui, ZH,&Qin, S.(2002).Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis.PROGRESS IN NATURAL SCIENCE,12(7),518-523.
MLA	Sun, X,et al."Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis".PROGRESS IN NATURAL SCIENCE 12.7(2002):518-523.

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