IOCAS-IR  > 实验海洋生物学重点实验室
Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization
Zhang Baoyu1; Chen Guofu2; Wang Guangce1,3; Lu Douding4
2010-03-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号28期号:2页码:310-314
文章类型Article
摘要A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant environmental problems. To forecast the occurrence of HAB, development of a rapid and precise detection method is urgently required. In this study, two Skeletonema costatum-like diatoms (SK-1 and SK-2), were identified morphologically under a light microscope, and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea, and strain SK-2 from an aquatic farm in Qingdao, China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including 5.8S rDNA) of strain SK-1. After hybridization, strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence microscope; however, no such fluorescence was observed with strain SK-2, which indicates that probes hybridized only the DNA of the target strain, SK-1, in species-specific manner, and that the two strains do not belong to a same species. This finding was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive, simple, and rapid, and is a promising tool for detecting target HAB species in natural environments.
关键词Fluorescent In Situ Hybridization Its Sequence Skeletonema Costatum Harmful Algal Bloom
DOI10.1007/s00343-010-9268-y
收录类别SCI
语种英语
WOS记录号WOS:000275781400016
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32860
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Harbin Inst Technol, Weihai 264209, Peoples R China
3.Tianjin Univ Sci & Technol, Tianjin 300457, Peoples R China
4.SOA, Inst Oceanog 2, Hangzhou 310012, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714
Zhang Baoyu,Chen Guofu,Wang Guangce,et al. Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(2):310-314.
APA Zhang Baoyu,Chen Guofu,Wang Guangce,&Lu Douding.(2010).Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(2),310-314.
MLA Zhang Baoyu,et al."Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.2(2010):310-314.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Identification of tw(841KB)期刊论文出版稿开放获取ODC PDDL浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang Baoyu]的文章
[Chen Guofu]的文章
[Wang Guangce]的文章
百度学术
百度学术中相似的文章
[Zhang Baoyu]的文章
[Chen Guofu]的文章
[Wang Guangce]的文章
必应学术
必应学术中相似的文章
[Zhang Baoyu]的文章
[Chen Guofu]的文章
[Wang Guangce]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。