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An inhibitor kappa B homologue from bay scallop Argopecten irradians
Mu, Changkao1,2; Yu, Yundong1; Zhao, Jianmin1; Wang, Lingling1; Song, Xiaoyan3; Zhang, Huan1; Qiu, Limei1; Song, Linsheng1
2010-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume28Issue:4Pages:687-694
SubtypeArticle
AbstractI kappa B is an important member of NF-kappa B pathway in the innate immune system. In the present study, the full-length cDNA sequence encoding I kappa B protein (designated AiI kappa B) was isolated from bay scallop Argopecten irradians. The complete sequence of AiI kappa B cDNA containing a 5' untranslated region (UTR) of 237 bp, a 3' UTR of 1023 bp with a poly (A) tail, and an open reading frame (ORF) of 1086 bp encoding a polypeptide of 361 amino acids with the predicted molecular weight of 39.9 kDa and theoretical isoelectric point of 4.7. Six ankyrin repeats which were necessary for specific binding to NF-kappa B and two potential phosphorylation sites responsible for I kappa B degradation were identified in the N-terminus of AiI kappa B. No PEST domain but a phosphorylation site motif (S(357)DSD(360)) was present at the C-terminus of AiI kappa B. Predicted three-dimensional structure of AiI kappa B shared high similarity with mammalian I kappa B alpha. Similarity and phylogenetic analysis revealed that AiI kappa B was clustered into I kappa Bs from invertebrate. All these typical characteristics indicated that the AiI kappa B should be classified into I kappa B family proteins. Quantitative real-time RT-PCR was employed to assess the mRNA expression of AiI kappa B in various tissues and its temporal expression in haemocytes of scallops challenged with Listonella anguillarum. The mRNA transcript of AiI kappa B could be detected in all the examined tissues with highest expression level in hepatopancreas. Bacteria infection inhibited the transcription level of AiI kappa B. The results suggested the involvement of AiI kappa B in responses against bacterial infection and further highlighted its functional importance in the immune system of A. irradians. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordArgopecten Irradians Innate Immunity Signaling Pathway Nf-kappa b i Kappa b Gene Cloning
DOI10.1016/j.fsi.2010.01.005
Indexed BySCI
Language英语
WOS IDWOS:000276935700023
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32851
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
3.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Mu, Changkao,Yu, Yundong,Zhao, Jianmin,et al. An inhibitor kappa B homologue from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(4):687-694.
APA Mu, Changkao.,Yu, Yundong.,Zhao, Jianmin.,Wang, Lingling.,Song, Xiaoyan.,...&Song, Linsheng.(2010).An inhibitor kappa B homologue from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,28(4),687-694.
MLA Mu, Changkao,et al."An inhibitor kappa B homologue from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 28.4(2010):687-694.
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