An inhibitor kappa B homologue from bay scallop Argopecten irradians

doi:10.1016/j.fsi.2010.01.005

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	An inhibitor kappa B homologue from bay scallop Argopecten irradians
	Mu, Changkao 1,2; Yu, Yundong 1; Zhao, Jianmin 1; Wang, Lingling 1; Song, Xiaoyan 3; Zhang, Huan 1; Qiu, Limei 1; Song, Linsheng 1
	2010-04-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	28 期号:4 页码:687-694
文章类型	Article
摘要	I kappa B is an important member of NF-kappa B pathway in the innate immune system. In the present study, the full-length cDNA sequence encoding I kappa B protein (designated AiI kappa B) was isolated from bay scallop Argopecten irradians. The complete sequence of AiI kappa B cDNA containing a 5' untranslated region (UTR) of 237 bp, a 3' UTR of 1023 bp with a poly (A) tail, and an open reading frame (ORF) of 1086 bp encoding a polypeptide of 361 amino acids with the predicted molecular weight of 39.9 kDa and theoretical isoelectric point of 4.7. Six ankyrin repeats which were necessary for specific binding to NF-kappa B and two potential phosphorylation sites responsible for I kappa B degradation were identified in the N-terminus of AiI kappa B. No PEST domain but a phosphorylation site motif (S(357)DSD(360)) was present at the C-terminus of AiI kappa B. Predicted three-dimensional structure of AiI kappa B shared high similarity with mammalian I kappa B alpha. Similarity and phylogenetic analysis revealed that AiI kappa B was clustered into I kappa Bs from invertebrate. All these typical characteristics indicated that the AiI kappa B should be classified into I kappa B family proteins. Quantitative real-time RT-PCR was employed to assess the mRNA expression of AiI kappa B in various tissues and its temporal expression in haemocytes of scallops challenged with Listonella anguillarum. The mRNA transcript of AiI kappa B could be detected in all the examined tissues with highest expression level in hepatopancreas. Bacteria infection inhibited the transcription level of AiI kappa B. The results suggested the involvement of AiI kappa B in responses against bacterial infection and further highlighted its functional importance in the immune system of A. irradians. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Argopecten Irradians Innate Immunity Signaling Pathway Nf-kappa b i Kappa b Gene Cloning
DOI	10.1016/j.fsi.2010.01.005
收录类别	SCI
语种	英语
WOS记录号	WOS:000276935700023
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32851
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 3.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Mu, Changkao,Yu, Yundong,Zhao, Jianmin,et al. An inhibitor kappa B homologue from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(4):687-694.
APA	Mu, Changkao.,Yu, Yundong.,Zhao, Jianmin.,Wang, Lingling.,Song, Xiaoyan.,...&Song, Linsheng.(2010).An inhibitor kappa B homologue from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,28(4),687-694.
MLA	Mu, Changkao,et al."An inhibitor kappa B homologue from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 28.4(2010):687-694.

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